What is EC50 in toxicology?

The EC50 (or ED50) is the concentration (or dose) effective in producing 50% of the maximal response and is a convenient way of comparing drug potencies. From: Medical Pharmacology and Therapeutics (Fifth Edition), 2018.

What does EC50 measure?

In pharmacology, the potency of a compound is expressed as the half-maximal effective concentration (EC50), which refers to the concentration of a drug that induces a response halfway between the baseline and maximum.

What is LD50 and EC50?

ED50: Median effective dose. The dose required to achieve 50% of the desired response in 50% of the population. EC50: Half maximum effective concentration. The concentration of a drug at which 50% of its maximum response is observed.

What is EC50 in Elisa?

EC50 or the concentration of antibody that gives half-maximal binding is determined by direct and saturable binding of a rAB dilution series to both target antigen and a non-specific control protein. An estimate of affinity is interpreted from one-half the concentration at which rAB binding first achieves saturation.

What does EC 50 mean in drug testing?

It is commonly used as a measure of a drug’s potency, and the use of EC 50 is preferred over that of ‘potency’, which has been criticised for its vagueness. EC 50 is a measure of concentration, expressed in molar units (M), where 1 M is equivalent to 1 mol/L.

What is the equation for EC50?

The EC 50 relates to the Hill equation, which is a function of the agonist concentration, [A]: E = E m a x 1 + ( EC 50 [ A ] ) n H {\\displaystyle E={\\frac {E_{\\mathrm {max} }}{1+\\left({\\frac {{\\ce {EC50}}}{{\\ce {[A]}}}}\\right)^{n_{H}}}}} [3]

When should I use the relative ec50/ic50 for assays?

Assays having a stable 100% control but for which there may be more than 5% error in the estimate of the 50% control mean should use the relative EC50/IC50.

What is EC50 of a graded dose response curve?

EC50 is expressed in molar units (M), where 1 M is equivalent to 1 mol/L. The EC50 of a graded dose response curve therefore represents the concentration of a compound where 50% of its maximal effect is observed.