What is derivatization in GC-MS?

Derivatization is the process of chemically altering an analyte or analytes. Laboratorians often choose to derivatize particular analytes in order to improve their chromatography, thermal stability, or their identification. Derivatization for GC-MS typically involves silylation, alkylation, or acylation reactions.

Can GC detect sugar?

The GC–MS method can detect all eight sugars and alditols in jujube extract, whereas that is five for the HPLC-ELSD method and six for the LC-ESI-MS/MS method.

Why sometimes derivatization is used in the sample preparation for GC-MS?

The derivatization is typically done to change the analyte properties for a better separation and also for enhancing the method sensitivity. In GC/MS, derivatization may improve the capability of compound identification.

How can glucose be derivatized?

In the quantitative analysis of sugars, such as glucose, performed using GC-MS, the sugars are derivatized in order to make them volatile. There are many derivatization methods, which can be classified according to reaction. They include acylation, esterification, and silylation.

What is derivatization in sample preparation?

Derivatization as a sample preparation technique is a well-known procedure used for making samples amenable for a chemical analysis or to improve the performance of analysis. The subject as a general procedure will be further presented in Section 13.7.

What is sample derivatization?

A change in the chemical form of the sample is sample derivatization, which is the focus of this column. Sample derivatization is a general term used for a chemical transformation designed to improve analytical capabilities, and it is a mainstay of analytical chemistry and instrumental analysis.

What are the methods of sugar determination?

The most commonly used methods for total sugar estimation are phenol sulfuric acid (PSA), 3-methyl-2-benzo thiazoline hydrazone hydrochloride (MBTH), and 2,4,6-tripyridyl-s-triazine (TPTZ).

What do you mean by chromatography how you will separate and identify the sugar present in the given mixture?

High-performance Liquid Chromatography (HPLC) As the solvent passes through it, different components interact with the solvent at different rates, leading to different flow rates. This leads to separation of the individual components. This method has been extensively used to analyze underivatized sugars.

What is derivatizing agent?

Derivatization Agents are used in the production of other compounds, such as primary amines and guanido groups, for use in a multitude of differing applications. Our popular products such as 2,3,4,5,6-Pentafluorobenzoyl chloride and p-Nitrophenylglyoxal are typically available for immediate shipment.

How derivatization is carried out in GC?

The three most widely used methods of derivatization in GC are silylation, acylation, and alkylation. Silylation. In this reaction, active hydrogens are displaced by a silyl group, most often tetramethylsilane (TMS). The general reaction scheme is illustrated for TMS reacting with an alcohol below.

How does derivatization work?

Derivatization is a technique used in chemistry which converts a chemical compound into a product (the reaction’s derivate) of similar chemical structure, called a derivative.