What is DDM detergent?

Product Description. n-Dodecyl-β-D-maltoside (DDM) is a non-ionic detergent commonly used to solubilize membrane-associated proteins.

What does maltoside Do?

Maltosides have been used extensively to stabilize membrane proteins for biophysical and structural studies.

What is the CMC of DDM?

DDM (dodecylmaltoside) has a CMC of 0.15 mM and a molecular weight of 510.6. A 1% solution (1 g/100 ml = 10 g/L) has a concentration of (10 g/L) x (mole/510.6 g) = 0.0196 M = 19.6 mM.

Is DDM an ionic detergent?

n-Dodecyl β-D-maltoside (DDM) is a non-ionic detergent commonly used during protein-anesthetic studies because of its mild and non-denaturing properties.

How do you store DDM?

Store at -20°C. Protect from light.

How do you store a DDM solution?

The 200x DDM/CHS solution should be diluted with assay buffer solutions to prepare 1x working solution just before experiment. Store this solution at 2-8℃, and protect from light. If precipitation occurs, filter to clarify before use. This Solution is stable after storage at 2-8℃ no less than 3 months upon receipt.

What is dodecyl maltoside?

Description. Dodecyl maltoside (DDM) is a non-ionic detergent that consists of a hydrophilic maltose head and a hydrophobic long chain alkyl tail. It has a relatively low critical micelle concentration of 0.17 mM and is considered a gentle but powerful detergent.

What is in detergent?

A detergent is a surfactant or a mixture of surfactants with cleansing properties when in dilute solutions. There are a large variety of detergents; often they are the sodium salts of long chain alkyl hydrogen sulphate or a long chain of benzene sulphonic acid.

How do you solubilize membrane proteins?

During the solubilization stage, membrane proteins are extracted from their natural environment, the lipid membrane, to an aqueous environment by the use of detergents. Detergents act by disintegrating the lipid bilayer while incorporating lipids and proteins in detergent micelles.

What is DDM in biology?

n-Dodecyl-B-D-Maltoside, or DDM, is a maltoside based non-ionic detergent with a hydrophilic maltose head and a hydrophobic long chain alkyl tail.

What are the two types of detergents?

Detergents are classified into four broad groupings, depending on the electrical charge of the surfactants.

  • Anionic detergents.
  • Cationic detergents.
  • Non-ionic detergents.
  • Amphoteric detergents.