What is counter immunoelectrophoresis test?

Counterimmunoelectrophoresis is a laboratory technique used to evaluate the binding of an antibody to its antigen, it is similar to immunodiffusion, but with the addition of an applied electrical field across the diffusion medium, usually an agar or polyacrylamide gel.

Why is counter immunoelectrophoresis called CIE?

Counter Current Immunoelectrophoresis is a modification of immunoelectrophoresis in which antigen and antibody move in opposite directions and form precipitates in the area where they meet in concentrations of optimal proportions. It is also referred to as countercurrent or crossed-over immunoelectrophoresis.

What is Rocket immunoelectrophoresis?

Rocket immunoelectrophoresis (also referred to as electroimmunoassay) is a simple, quick, and reproducible method for determining the concentration of a specific protein in a protein mixture.

What is electro immunodiffusion?

[ ĭ-lĕk′trō-ĭm′yə-nō-dĭ-fyōō′zhən, -ĭ-myōō′- ] n. An immunochemical method that combines electrophoretic separation with immunodiffusion by incorporating antibody into the support medium. GOOSES.

Why CFT test is done?

The fungal antigens and positive controls are used to detect antibodies in patient serum by the complement fixation (CF) procedure to aid in the diagnosis of four specific fungal diseases : Histoplasmosis, blastomycosis, coocidioidomycosis and aspergillosis.

How is immunofixation done?

Technique. The technique consists of depositing a serum (or urine which has been previously concentrated) sample on a gel. After application of an electric current that allows the separation of proteins according to their size, antibodies specific for each type of immunoglobulin are laid upon the gel.

What is Ria in microbiology?

Radioimmunoassays. Radioimmunoassay (RIA) is a competitive assay technique in which the reagent, the antibody (Ab), is used in a limited amount as compared with the amount of analyte antigen (Ag). To perform a RIA, it is essential to have a radioactively labeled antigen (Ag∗), which also is referred to as the tracer.

What are the applications of Rocket immunoelectrophoresis?

Applications of Rocket Immunoelectrophoresis Used in enzyme activity electrophoresis. Studies dealing with antigenic relationships between organisms. Used to Determine the concentration of a specific protein in a protein mixture. Used for the estimation of immunoglobulin protease activity.

Who discovered immunoelectrophoresis?

The antibodies diffuse laterally to meet diffusing antigens, and lattice formation and precipitation occur permitting determination of the nature of the antigens. The term “immunoelectrophoresis” was first coined by Grabar and Williams in 1953.

What is radioimmunoassay technique?

Radioimmunoassay technique (RIA) is a very sensitive in vitro technique used to measure the concentration of antigens (eg, hormone levels in the blood) through the use of antibodies directed against these antigens.

What radioimmunoassay means?

Radioimmunoassay: A very sensitive, specific laboratory test (assay) that uses radiolabeled and unlabeled substances in an immunological (antibody-antigen) reaction.

What is CFT blood test?

The complement fixation test (CFT) is a classical laboratory diagnostic test, which is still used for determination of virus antibodies in patient sera or cerebrospinal fluid samples during an acute infection. The test mainly measures IgG antibodies.