What is considered high throughput screening?
What is considered high throughput screening?
High-throughput screening (HTS) is a drug discovery process that allows automated testing of large numbers of chemical and/or biological compounds for a specific biological target, for example through binding assays.
What is the objective of high throughput screening?
The objective of HTS is to rapidly identify active compounds that modulate a particular target, pathway, or biochemical/cellular event.
What is high content imaging?
High content imaging applies automated microscopy, fluorescent detection, and multiparameter algorithms to visualize and quantify the interaction of therapeutics in cell populations.
What is virtual screening how does it differ from high-throughput screening?
Virtual screening, similar to HTS, relies upon assessing a large, diverse library of compounds. The difference is that the compounds in a virtual screening library are contained in databases rather than being physically assembled by the organisation conducting the screen.
What is virtual screening how does it differ from high throughput screening?
What is HTC in cloud computing?
7.1. High-throughput computing (HTC) is the use of distributed computing facilities for applications requiring large computing power over a long period of time. HTC systems need to be robust and to reliably operate over a long time scale.
How do you get high-throughput screening?
High-throughput Screening Steps
- Consultation. Potential users are invited to discuss a screening idea with any member of the SMDC HTS group.
- Assay development. The assay is developed by the initiating lab, usually in their laboratory.
- Dry-run.
- Pilot screen.
- HTS.
- Data analysis.
- Rescreen.
- Preliminary SAR.
What is a target in drug discovery?
A drug target is a molecule in the body, usually a protein, that is intrinsically associated with a particular disease process and that could be addressed by a drug to produce a desired therapeutic effect.
What defines high-throughput and high-content screens?
High-throughput screening (HTS) of small molecules allows the rapid interrogation of the effects of thousands to hundreds of thousands of small molecules in a variety of in vitro and cell-based assays, whereas high-content screening (HCS) approaches may sacrifice some of these high-throughput capabilities in return for …
What is HCS biology?
Abstract. High-content screening (HCS, also known as high-content analysis, or HCA) is an approach to cell biology that combines automated imaging and quantitative data analysis in a high-throughput format suitable for large-scale applications such as drug discovery and systems biology.
