What is buffered peptone water used for?

Buffered Peptone Water is a non-selective pre-enrichment medium. It also provides conditions for resuscitation of cells that have been injured by processes of food preservation. Low numbers and/or sub-lethally injured Salmonella spp. cells may be present in a test food sample.

How do you store buffered peptone water?

Buffered Peptone Water may be used according to published standard methodologies. To comply with ISO methodology, please use Buffered Peptone Water (ISO) (CM1049) and see relevant ISO standard8. Store dehydrated medium at 10-30°C and use before the expiry date of the label.

What is the pH of buffered peptone water?

pH 7.0
Buffered Peptone Water (ISO), pH 7.0 is used for the non-selective pre-enrichment of Salmonella spp. from food in a laboratory setting.

Is Peptone Water selective?

Alkaline Peptone Water was first formulated by Shread, Donovan and Lee1 to be used as a non-selective enrichment broth for the cultivation of Aeromonas spp.

Why is Peptone Water used for dilution?

The pH of this diluent medium is near neutral range optimum for viability of microorganisms. Therefore it can be successfully used as a diluent for carrying out dilutions of different samples.

Why peptone is used in culture media?

The addition of peptone provides a readily available source of nitrogen and carbon. Peptone is used in culture media to mainly supply nitrogen. Most organisms are capable of utilizing the amino acids and other simpler nitrogenous compounds present in peptone.

What is the purpose of the buffered peptone water pre-enrichment step in the isolation of Salmonella from food?

Buffered Peptone Water is a pre-enrichment medium used for sample preparation from foods prior to selective enrichment and isolation. Pre-enrichment in a non-selective medium allows for repair of cell damage and facilitates the recovery of Salmonella.

What is BPW media?

SampleReady® Soluble Pre-Weigh Media Pouch. Buffered Peptone Water (BPW) is used as a pre¬enrichment medium for the isolation of Salmonella sp. from food products, particularly injured microorganisms.

How do you make 0.1 buffered peptone water?

0.1% Peptone Salt Solution is used as diluent for different test method. Suspend 9.50 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes i.e. validated cycle.

Why are peptones used in media preparation?

Peptones are widely used as supplements for serum-free culture media. The growth-promoting activity of peptones may have a dual effect in batch cultures, while it may promote rapid cell growth in the first days of culture.

Is peptone water soluble?

Peptone water is a buffered solution generally containing peptone—a soluble protein—which is then buffered to a neutral pH (approximately 7.0) using a base disodium phosphate.

Why is peptone water used for dilution?