What is BrdU staining?
What is BrdU staining?
BrdU Staining and Labeling Protocols BrdU (5′-bromo-2′-deoxyuridine) is a thymidine analog that has been established as a popular tool in cancer and neuroscience research for determining cell proliferation rates.
What color is BrdU?
BrdU Cell Proliferation Assay Kit 6813
| Product Includes | Quantity (with Count) | Solution Color |
|---|---|---|
| BrdU | 1 x 150 µl | |
| Fixing/denaturing Solution | 2 x 25 ml | |
| BrdU Mouse Detection mAb | 1 x 500 µl | Green |
| Anti-mouse IgG, HRP-Linked Antibody | 1 x 500 µl | Red |
What does BrdU assay measure?
Bromodeoxyuridine (BrdU) incorporation assays have long been used to detect DNA synthesis in vivo and in vitro. The key principle of this method is that BrdU incorporated as a thymidine analog into nuclear DNA represents a label that can be tracked using antibody probes.
Why is BrdU used?
Bromodeoxyuridine (BrdU) is a thymidine analog that incorporates DNA of dividing cells during the S-phase of the cell cycle. As such, BrdU is used for birth dating and monitoring cell proliferation.
What kind of cells does BrdU stain?
Bromodeoxyuridine (BrdU) is one such common nucleotide analog that is classically used to track label‐retaining cells (LRCs) after a prolonged “washout” period that dilutes the label within the more rapidly cycling transient‐amplifying (TA) cells.
What is BrdU a marker of?
BrdU is not a marker of the S-phase of the cell cycle. As a thymidine analog, it is a marker of DNA synthesis.
How do you stain BrdU?
Detect incorporated BrdU
- Remove this solution and add 1 mL of antibody staining buffer.
- Add anti-BrdU primary antibody.
- Incubate overnight at room temperature.
- Wash with Triton X-100 permeabilization buffer (3 times, 2 minutes each)
- Add fluorescently labeled secondary antibody.
- Incubate one hour at room temperature.
What is BrdU positive?
BrdU-positive cells without co-labeling and BrdU/RCA-positive cells occur first after the H-I injury (significant increase at P10), followed by BrdU/NeuN-positive cells (significant increase at P42). Colabeling of BrdU and NeuN was confirmed by confocal microscopy (Figure 2).
Is BrdU an antibody?
Specificity. BrdU is a thymidine analogue and when offered to proliferating cells it is incroporated into reduplicating cells. The antibody is specific for DNA in which BrdU has been incorporated.
