What is anion exchange purification?

Anion exchange chromatography is commonly used to purify proteins, amino acids, sugars/carbohydrates and other acidic substances with a negative charge at higher pH levels. The tightness of the binding between the substance and the resin is based on the strength of the negative charge of the substance.

What is anion exchange process?

Anion exchange is the process in which anions in the form of acids are adsorbed by a basic substance. It describes the exchange of ions in which one anion (as chloride or hydroxide) is substituted for one or more other anions (as sulfate). It is highly effective on negatively charged ions such as: Bicarbonate.

What does an anion exchange column do?

Anion Exchange Column Design For molecules with multiple charged groups, such as proteins, it is the overall charge in a given buffer chemistry (pH, etc.) that determines whether and how strongly the molecule will bind to the column stationary phase.

How does anion chromatography work?

Anion exchange chromatography is a form of ion exchange chromatography (IEX), which is used to separate molecules based on their net surface charge. Anion exchange chromatography, more specifically, uses a positively charged ion exchange resin with an affinity for molecules having net negative surface charges.

Which types of molecules elute first in anion exchange chromatography?

For example, when cation exchange chromatography is used, cations will elute out last. Meanwhile, the negative charged molecules will elute out first.

What is the difference between cation and anion resin?

Anion resins and cation resins are the two most common resins used in the ion-exchange process. While anion resins attract negatively charged ions, cation resins attract positively charged ions.

Why resin is used in ion exchange chromatography?

Ion exchange resins are used to soften water by replacing the cations with sodium ions (and possibly the anions with chloride ions) of sodium chloride. They may also be used to demineralize water where the cations are replaced by H+ ions and the anions are replaced by OH− ions.