What is a Bicistronic gene?

As the name suggests, bicistronic plasmids contain two distinct genes of interest within one vector. The vector transports the genes together into the cells, which means that every cell with one gene also has the other.

Can a single mRNA encode more than 1 protein?

Death of a dogma: eukaryotic mRNAs can code for more than one protein.

What is the purpose of the cap and tail?

The group at the beginning (5′ end) is called a cap, while the group at the end (3′ end) is called a tail. Both the cap and the tail protect the transcript and help it get exported from the nucleus and translated on the ribosomes (protein-making “machines”) found in the cytosol 1start superscript, 1, end superscript.

What is a Bicistronic expression vector?

IRES-containing bicistronic vectors allow the simultaneous expression of two proteins separately, but from the same RNA transcript (Jackson et al. 1990; Jang et al. 1988).

How does t2a peptide work?

The 2A peptides can be applied when the fused protein doesn’t work. Inserting the CDS of a 2A peptide into the fusing point or replacing the linker sequence with the CDS of a 2A peptide protein can cleave the fused protein into two separated peptides, making the two peptides to regain the function.

How many proteins can a single mRNA make?

Another fascinating result was that proteins were about 900 times more abundant than the mRNAs used to make them – one way to think of this is that on the average, a single mRNA is used to manufacture about 900 copies of the corresponding protein.

How many times can a single mRNA molecule be translated into protein?

However, errors occurring during transcription often elicit more dire consequences than those occurring during translation because individual mRNAs can be translated up to 40 times (primary sources), resulting in a burst of flawed proteins.

https://www.youtube.com/watch?v=8_f-8ISZ164