What does the Gal80 gene do?

In the absence of galactose, Gal80 binds to and inhibits the transcriptional activation domain (AD) of the GAL gene activator, Gal4, preventing GAL gene expression. Galactose triggers an association between Gal3 and Gal80, relieving Gal80 inhibition of Gal4.

How does Gal80 inhibit GAL4?

In the absence of galactose, the Gal80 protein binds to a small peptide (amino acids [aa] 855 to 870) within the Gal4AD and thereby prevents Gal4-mediated promoter activation (10, 11).

Does Gal3 bind Gal80?

Gal3 Binds Gal80 Tighter than Gal1 Indicating Adaptive Protein Changes Following Duplication.

Is Gal80 a repressor?

GAL80 encodes a transcriptional repressor involved in transcriptional regulation in response to galactose (2).

What does GAL80 bind to?

In the absence of galactose, Gal80 binds to and inhibits the transcriptional activation domain (AD) of the GAL gene activator, Gal4, preventing GAL gene expression.

What is the consequence of DNA methylation?

DNA methylation, a process of adding a methyl group to DNA done by a DNA methyltransferase is a heritable (epigenetic) alteration leading to cancer, atherosclerosis, nervous disorders (Imprinting disorders), and cardiovascular diseases.

How does GAL4 work?

Gal4 is a transcriptional activator that binds to UAS enhancer sequences found in DNA. It then recruits transcription machinery to the site to induce gene expression. Thus, genes and siRNA encoded downstream of the UAS sequence are only expressed when Gal4 is expressed.

What does Gal80 bind to?

How does Gal4 work?

What is Gal4p?

Gal4p mediates expression of most of the genes involved in utilization of galactose, a relatively poor carbon source that is used only if glucose or other carbon sources are not available.

How can Micrornas Mirnas regulate gene expression?

microRNA controls gene expression mainly by binding with messenger RNA (mRNA) in the cell cytoplasm. Instead of being translated quickly into a protein, the marked mRNA will be either destroyed and its components recycled, or it will be preserved and translated later.

How does DNA methylation cause gene silencing?

DNA methylation regulates gene expression by recruiting proteins involved in gene repression or by inhibiting the binding of transcription factor(s) to DNA.