What does dUTPase do?
What does dUTPase do?
Two enzyme activities are primarily involved in keeping DNA uracil-free: dUTPase (dUTP pyrophosphatase) activity that prevent thymine-replacing incorporation and uracil-DNA glycosylase activity that excise uracil from DNA and initiate uracil-excision repair.
What happens once the retrovirus is inside the cell?
After a retrovirus enters a host cell, reverse transcriptase converts the retroviral RNA genome into double-stranded DNA. This viral DNA then migrates to the nucleus and becomes integrated into the host genome.
Is reverse transcriptase found in retroviruses?
The enzyme reverse transcriptase (RT) was discovered in retroviruses almost 50 years ago. The demonstration that other types of viruses, and what are now called retrotransposons, also replicated using an enzyme that could copy RNA into DNA came a few years later.
What does dUTP stand for?
dUTP
| Acronym | Definition |
|---|---|
| dUTP | 2′-Deoxyuridine 5′-Triphosphate |
| dUTP | Dhaka Urban Transport Project (World Bank; Bangladesh) |
What makes a retrovirus different from other viruses?
Retroviruses differ from other viruses in that each virion contains two complete copies of the single-stranded RNA genome.
What is the function of reverse transcriptase in retrovirus?
What is the function of reverse transcriptase in retroviruses? As mentioned, reverse transcriptase allows viruses to convert their RNA genome into DNA so that it can be inserted into the host’s genome.
What is the role of reverse transcriptase in retroviruses?
Reverse transcriptase is an enzyme found in retroviruses that converts the RNA genome carried in the retrovirus particle into double-stranded DNA. Reverse transcriptase first transcribes a complementary strand of DNA to make an RNA:DNA hybrid.
What is dUTP and dTTP?
dUTP (2´-Deoxyuridine, 5´-Triphosphate) can be used in place of dTTP in PCR and RT-PCR protocols to prevent carryover from previous amplifications. The substitution of dUTP for dTTP in PCR results in uracil-containing PCR products that are suitable for most standard applications.
