What does alcian blue stain for?
What does alcian blue stain for?
Alcian Blue is a stain that is used to visualize acidic epithelial and connective tissue mucins. Mucins are a type of carbohydrate and are found in the GI tract and respiratory tract. Acidic mucins have a negative charge.
How do you dissolve Alizarin Red?
Alizarin Red S Solution preparation: Dissolve 2 g Alizarin Red S (Sigma, Cat# A5533) in 100 ml of ddH2O and adjust pH to 4.1-4.3 with HCl or NH4OH. Filter the solution through 0.22 µ membrane and store at 4oC in dark. 2. Remove the Osteogenic Differentiation Medium.
What stains cartilage blue?
Alizarin red/alcian blue staining of a newborn skeleton. Ossified tissue (predominantly bone) stains red and cartilage stains blue. The soft tissues are cleared in an alkaline solution and glycerol. (Image courtesy of Kazuhisa Nakashima, University of Texas M.D. Anderson Cancer Center.)
What is alizarin used for?
Alizarin (also known as 1,2-dihydroxyanthraquinone, Mordant Red 11, C.I. 58000, and Turkey Red) is an organic compound with formula C14H8O4 that has been used throughout history as a prominent red dye, principally for dyeing textile fabrics.
What does alcian blue stain in cartilage?
Alcian blue and Alizarin red stain cartilage and bone, respectively. As a cationic dye, Alcian blue binds strongly to sulfated GAGs and glycoproteins, while Alizarin red, an anionic dye, binds to cationic metals such as calcium [1].
Is alcian blue a special stain?
Alcian Blue is a basic dye containing copper, which gives it the blue color. The stain molecules carry a positive charge and are attracted to the negative mucins….1) Alcian Blue.
| Tissue type | GI tract |
|---|---|
| Composition | Neutral mucins |
| Alcian blue 2.5 | Negative |
| Alcian blue 1.0 | Negative |
How do you make Alcian blue stain?
Dissolve the dye in the distilled water, add acid, mix well. Filter into the reagent bottle and filter before use. Dissolve 1 gm of alcian blue in 90mls of distilled water and 10mls of 1N hydrochloric acid.
How do you prepare an osteogenic medium?
Finally, the experimental osteogenic medium was prepared by adding 10% FBS, 0.01 μM dexamethasone, 50 μg/mL ascorbic acid, 10 mM sodium β-glycerophosphate, 10,000 U/mL penicillin, and 10,000 U/mL streptomycin to the experimental culture medium.
Why does Alcian blue stain cartilage?
As a cationic dye, Alcian blue binds strongly to sulfated GAGs and glycoproteins, while Alizarin red, an anionic dye, binds to cationic metals such as calcium [1]. Because cartilage contains higher concentrations of GAGs than any other tissue, it binds more Alcian blue.
