What are the two types of molecular markers?

The three most common types of markers used today are RFLP, RAPD and isozymes. Of the three marker types, RFLPs have been used the most extensively.

What is the purpose of studying isozymes analysis?

Isozyme analysis is a powerful biochemical technique with numerous applications in plant pathology. It has long been used by geneticists to study the population genetics of fish, mammals, insects, nematodes, and higher plants.

What are the different types of molecular markers?

Molecular markers-types and applications

  • amplified fragment length polymorphism.
  • expressed sequence tags.
  • genetic markers.
  • inter simple sequence repeat markers.
  • microsatellites.
  • molecular markers.
  • random amplified polymorphic DNA.
  • restriction fragment length polymorphism.

What are dominant and co dominant markers?

Codominant markers indicate differences in size whereas dominant markers are either present or absent. Strictly speaking, the different forms of a DNA marker (e.g. different sized bands on gels) are called marker ‘al- leles’.

What is SSR marker?

Microsatellites, otherwise called Simple sequence repeats (Ssrs) or Short Tandem Repeats (Strs), are rehashing sequences of 2-5 base sets of Dna.it is a sort of Variable Number Tandem Repeat (VNTR). Microsatellites are commonly co-prevailing.

What are isozymes and discuss their functions?

Isozymes (also known as isoenzymes) are enzymes that differ in amino acid sequence but catalyze the same chemical reaction. These enzymes usually display different kinetic parameters (i.e. different KM values), or different regulatory properties.

What are isozymes discuss their significance?

In biochemistry, isozymes (also known as isoenzymes or more generally as multiple forms of enzymes) are enzymes that differ in amino acid sequence but catalyze the same chemical reaction. Isozymes usually have different kinetic parameters (e.g. different KM values), or are regulated differently.

What is co marker?

Codominant markers are markers for which both alleles are expressed when co-occurring in an individual. Therefore, with codominant markers, heterozygotes can be distinguished from homozygotes, allowing the determination of genotypes and allele frequencies at loci.

Why SSR markers are codominant?

Characteristics: SSRs are single locus markers and have a few to even more than ten alleles in each locus; thus, SSRs are highly polymorphic. SSRs are co-dominant markers so they can distinguish heterozygotes from homozygotes. The main advantages of SSRs are their high level of polymorphism and their reliability.

What is the difference between SSR and ISSR markers?

SSR markers showed a mean PIC value of 0.78 hence the best marker. As RAPD and ISSR markers are biallelic in nature, they can have maximum of 0.50 PIC value. But SSR markers are multi-allelic and their PIC values will be ranging from 0 if it is monomorphic to 1 if it is highly discriminative.

Why SSR markers are important?

SSRs play a major role as molecular markers for genome analysis and plant breeding. The microsatellites existing in the complete genome sequences would have a direct role in the genome organization, recombination, gene regulation, quantitative genetic variation, and evolution of genes.