What are the mouse MHC haplotypes?

MHC molecules are highly polymorphic. Laboratory mice are inbred so that each strain is homo- zygous and has a unique MHC haplotype. The MHC haplotype in these strains is designated by a small letter (a, b, d, k, q, s, etc.). For example, MHC haplotype antigens of BALB/c mice are H-2Kd, H-2Dd, H- 2Ld, I-Ad, and I-Ed.

Where are the class I and II MHC molecules Synthesised?

MHC class I molecules acquire peptides right after synthesis in the endoplasmic reticulum. MHC class II molecules do not acquire peptides in the endoplasmic reticulum but instead associate with a third chain, the invariant chain which impedes peptide binding.

How many MHC haplotypes are there?

Sequences of 95 human MHC haplotypes reveal extreme coding variation in genes other than highly polymorphic HLA class I and II.

How many classes of MHC I are expressed on a single cell?

Each human cell expresses six MHC class I alleles (one HLA-A, -B, and -C allele from each parent) and six to eight MHC class II alleles (one HLA-DP and -DQ, and one or two HLA-DR from each parent, and combinations of these).

What is the meaning of haplotype?

A haplotype is a group of genes within an organism that was inherited together from a single parent. The word “haplotype” is derived from the word “haploid,” which describes cells with only one set of chromosomes, and from the word “genotype,” which refers to the genetic makeup of an organism.

Why is MHC polymorphic?

Since MHC molecules play an important role in the induction of immune responses, this polymorphism is probably due to selection for increased protection of hosts against pathogens. In contrast to the large population diversity of MHC molecules, each individual expresses only a limited number of different MHC molecules.

Where are MHC 1 molecules found?

cell surface
MHC class I molecules are one of two primary classes of major histocompatibility complex (MHC) molecules (the other being MHC class II) and are found on the cell surface of all nucleated cells in the bodies of vertebrates. They also occur on platelets, but not on red blood cells.

What is the difference between MHC class 1 and 2?

The main difference between MHC class 1 and 2 is that MHC class 1 molecules present antigens to cytotoxic T cells with CD8+ receptors whereas MHC class 2 molecules present antigens to helper T cells with CD4+ receptors.

Where is MHC 1 found?

Do all cells express MHC class I?

Classical MHC class I molecules are ubiquitously expressed on all mammalian cells including cells of epithelial origin while MHC class II molecules are selectively expressed on antigen-presenting cells (APC) including dendritic cells (DC), macrophages, and B cells.

Where are MHC class I molecules found?

What is a C57BL/6 mouse?

The C57BL/6 mouse is the second mammal to have complete genome sequencing (after humans), and the International Mouse Phenotyping Association (IMPC) has since been performing functional analysis of its genes. As an inbred strain, mice of the C57BL/6 background share a nearly identical genotype.

What is the difference between C57BL/6 and BALB/c?

The Immunological Differences Between C57BL/6 and BALB/c. C57BL/6 and BALB/c differ in their innate immune responses, primarily between type 1 and type 2 helper T cells (Th1 and Th2). In C57BL/6 mice, Th1 immune response and IFNγ production play the key role.

What does MHC class I stand for?

Peritoneal macrophages from C57BL/6 mice process antigens from bacteria or coated on polystyrene beads for presentation by major histocompatibility complex (MHC) class I molecules.

Does the monoclonal antibody react with H-2Kb MHC?

Description: This AF6-88.5.5.3 monoclonal antibody reacts with the H-2Kb MHC class I alloantigen. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g. d, f, j, k, p, q, r, s, u, and v) has not been observed.