What are ribosome protected fragments?

One technique is to isolate and sequence the nuclease-resistant ribosome-protected fragments or footprints (RPFs), which comprise the approximately 30 nucleotides of mRNA bound to the ribosome during translation.

What is Translatome analysis?

Translatomics is the study of all open reading frames (ORFs) that are being actively translated in a cell or organism. This collection of ORFs is called the translatome. Characterizing a cell’s translatome can give insight into the array of biological pathways that are active in the cell.

What is deep sequencing technique?

Deep sequencing refers to sequencing a genomic region multiple times, sometimes hundreds or even thousands of times. This next-generation sequencing (NGS) approach allows researchers to detect rare clonal types, cells, or microbes comprising as little as 1% of the original sample.

What is rip sequencing?

RIP-Seq maps the sites at which proteins are bound to the RNA within RNA-protein complexes. In this method, RNA-protein complexes are immunoprecipitated with antibodies targeted to the protein of interest. After RNase digestion, RNA protected by protein binding is extracted and reverse-transcribed to cDNA.

What is trap seq?

Targeted purification of polysomal mRNA (TRAP-Seq) maps translating mRNAs under various conditions. In this method, tagged ribosomal proteins are expressed in cells. The tagged ribosomal proteins are purified and the RNA isolated. The RNA is reverse-transcribed to cDNA.

Why is called deep sequencing?

Deep sequencing refers to sequencing a genomic region multiple times, sometimes hundreds or even thousands of times.

Why is deep sequencing important?

Deep sequencing yields a unique genetic fingerprint that can be used to identify a person, and a trove of predictors of genetic medical diseases. Deep sequencing to identify epigenetic events including changes in DNA methylation and RNA expression can reveal the history and impact of environmental exposures.