What are MCF-7 cells used for?

MCF-7 cells are useful for in vitro breast cancer studies as a result of the cell line retaining several ideal characteristics particular to the mammary epithelium. These include the ability for MCF-7 cells to process estrogen in the form of estradiol via estrogen receptors in the cell cytoplasm.

What type of cell is MCF-7?

MCF7 are epithelial cells isolated from the breast tissue of a 69-year-old, White, female patient with metastatic adenocarcinoma. Use these cells in your breast cancer research. Discounts are available for our fellow nonprofit organizations.

What are MCF-7 ADR cells?

Abstract. MCF-7/ADR cells have been widely used as a multidrug-resistant breast cancer cell model in cancer research. The origin of MCF-7/ADR has been a matter of debate since MCF-7/ADR cells were re-designated NCI/ADR-RES in 1998.

How do you grow MCF-7 cells?

The common medium for culturing MCF-7 cells is Eagle’s MEM (EMEM), supplemented with 10% FBS and 1% penicillin/streptomycin, non essential amino acids (0.1 mM), insulin (10 ug/mL) and sodium pyruvate (1 mM). Add 10 nM estrogen to media for a 3-4 x increase in cell population growth.

Do MCF7 cells express HER2?

Nevertheless, MCF-7 are considered to be moderate EGFR-expressing cell lines (2). Discoveries in breast cancer pathology through experiments using MCF-7 cells. MCF-7 sub-lines demonstrate a wide divergence in the relative expression of ER, PR and HER2.

What is the difference between MCF7 and MDA-MB-231?

The MCF-7 cell line has functional estrogen and EGF receptors, is dependent on estrogen and EGF for growth, and is uninvasive, while MDA-MB-231 cells are a model for more aggressive, hormone-independent breast cancer.

Are MCF-7 cells adherent?

MCF-7 is an adherent, epithelial luminal cell line positive for estrogen and progesterone receptors. Although they are referred to as hormone-dependent, the minimal amount of steroid hormones present in serum is sufficient to ensure cell growth.

Are MCF-7 cells metastatic?

MCF-7 is a poorly-aggressive and non-invasive cell line (9), normally being considered to have low metastatic potential (8).

How do you split MCF-7 cells?

Add 2-3 mL of warm (37°C) 0.25% Trypsin- 0.53 mM EDTA solution to cells to detach the cell layer. Observe cells under an inverted microscope. Dispersal should happen between 5 and 15 minutes. If cells are not detaching properly, place flask back in 37°C incubation chamber.

Is MCF-7 invasive?

Though MCF7 is categorized as poorly invasive, its migration in the 2D surface is found to be comparable to the highly invasive MDA MB 231 in the current study.