How do you calculate TM for primers?

How do you calculate TM for primers? The equation used for the melting temperature is: Tm = 81.5 + 0.41(%GC) – 675/N – % mismatch, where N = total number of bases. How do you calculate annealing temperature of Tm primers? The optimal annealing temperature (Ta Opt) for a given primer pair on a particular […]

How do I ignore untracked files in git?

How do I ignore untracked files in git? You can use the git clean command to remove untracked files. The -fd command removes untracked directories and the git clean -fx command removes ignored and non-ignored files. Why is Gitignore in untracked files? gitignore file is showing up on the status, because it’s untracked, and git […]

Who is George Kristiansen?

Who is George Kristiansen? George Robert Christensen (born 30 June 1978) is an Australian politician and former journalist who was a member of the Australian House of Representatives from 2010 to 2022, as the member of parliament (MP) for the division of Dawson. Where is Wyatt Roy now? Post political career Roy later joined Afiniti, […]

Who created the P1?

Who created the P1? Ferdinand Porsche The Porsche P1, otherwise known as the Egger-Lohner electric vehicle, C. 2 Phaeton model, is the first Porsche vehicle. It was designed by Ferdinand Porsche and is considered to be the first stepping stone for him before he and his son created the company, Porsche…. Porsche P1 Curb weight […]

Can you stream ps2 emulator on Twitch?

Can you stream ps2 emulator on Twitch? It is perfectly fine to use an emulator on Twitch. The use of emulators is very popular, especially within the speedrunning and retro game communities. The real issue lies in the use of playing specific game ROMs. It is not only against Twitch TOS to play a cracked […]

How do you unclog a waste stack?

How do you unclog a waste stack? How to Clean and Clear Your Vent Climb onto your roof. Have an assistant flush a toilet while you hold your hand over the vent. Use a plumber’s snake of electricians fish tape to unclog the stoppage. If you can’t entirely remove the clog with your “snake,” use […]

What is profit maximization concept?

What is profit maximization concept? Profit maximisation is a process business firms undergo to ensure the best output and price levels are achieved in order to maximise its returns. Influential factors such as sale price, production cost and output levels are adjusted by the firm as a way of realising its profit goals. What is […]

What is the definition of a grow op?

What is the definition of a grow op? grow-op in British English noun. Canadian. an illegal scheme to grow marijuana plants. How do you start a grow op? The specific steps you’ll need to take during this stage of planning your first indoor grow are: Setting up the grow room ventilation. Routing your water supply […]

Is Moriarty in Hound of the Baskervilles?

Is Moriarty in Hound of the Baskervilles? That scene of Moriarty in prison at the end of “The Hounds of Baskerville” isn’t there just for the sake of having an extra something, but to show how far Moriarty’s obsession with Sherlock went, as he scratched his name on the walls and the two-way mirror. Is […]

What is difference between monodisperse and polydisperse?

What is difference between monodisperse and polydisperse? Monodisperse polymers are macromolecular materials having a precise and discrete molecular weight while polydisperse polymers are macromolecular materials having a range of components with a range of molecular weights. What is polydispersity and Monodispersity? Monodisperse polymers are uniform polymers in which all molecules have the same degree of […]

What are some cool chemical names?

What are some cool chemical names? Name based on shape Barrelene C8H8, the name derives from the resemblance to a barrel. Olympicene Refers to the fused 5-benzene rings (C19H12), which is reminiscent of the Olympic Flag. Penguinone 3,4,4,5-tetramethylcyclohexa-2,5-dienone; a two-dimensional representation of its structure resembles a penguin. What is the chemical composition of a cat? […]

What does the Get Up Go test assess?

What does the Get Up Go test assess? The Timed Up and Go test (TUG) is a simple test used to assess a person’s mobility and requires both static and dynamic balance. It uses the time that a person takes to rise from a chair, walk three meters, turn around 180 degrees, walk back to […]