Is Platinum Taq High Fidelity?

Platinum® Taq DNA Polymerase, High Fidelity, is ideal for amplification of DNA fragments when high yields and robust amplification are required.

Does Platinum Taq have proofreading?

Platinum Taq DNA Polymerase is composed of recombinant Taq DNA Polymerase, Pyrococcus GB-D Polymerase and Platinum Taq Antibody. Pyrococcus GB-D Polymerase possesses proofreading ability and (when mixed with regular Taq Polymerase) increases fidelity by approximately six fold.

What is the function of Taq polymerase?

Taq polymerase is the heat-stable (thermostable) DNA polymerase extracted from the thermophilic bacteria Thermus aquaticus. Its predominant function is in the polymerase chain reaction (PCR) technique, where it automates the repetitive step of amplifying specific DNA sequences.

Is Taq polymerase High Fidelity?

One unit of Platinum™ Taq DNA Polymerase, High Fidelity, is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.

What do you understand with high fidelity and thermostable DNA polymerase?

High-fidelity DNA polymerases have several safeguards to protect against both making and propagating mistakes while copying DNA. Such enzymes have a significant binding preference for the correct versus the incorrect nucleoside triphosphate during polymerization.

What is the error rate of Taq polymerase?

For Taq polymerase, which has a total error rate of 1.8 × 10−4 errors/base/doubling, single-molecule sequencing was sufficient to detect all types of polymerase errors, including base substitutions, deletions and insertions.

What are the advantages of Taq polymerase?

Thermostability: Taq polymerase is a thermostable DNA polymerase isolated from a bacterium that lives in hot springs. It can withstand the high temperature of >90°C required for the denaturing step in PCR and remain enzymatically active after each cycle.

What makes Taq polymerase unique?

Taq DNA Polymerase is highly efficient, so it becomes fully functional as it reaches its optimum temperature. It also has a half-life of more than two hours (at a temperature of 92 °C), a high-amplification capacity, and the ability to add 150 nucleotides per second.

What is the source of Taq polymerase?

thermophilic bacterium Thermus aquaticus
Taq polymerase is a thermostable DNA polymerase I obtained from the thermophilic bacterium Thermus aquaticus.

What is better than Taq polymerase?

Conclusion. High-fidelity DNA polymerases offer a highly accurate alternative to standard Taq polymerase when using PCR to produce amplicons for sensitive downstream applications such as cloning and sequencing.