Is Mueller-Hinton agar selective or differential?

non-selective
Mueller-Hinton agar is a non-selective, non-differential microbiological growth medium. Mueller Hinton Agar contains beef extract, acid casein hydrolysate, starch and agar. Beef extract and casein acid hydrolysate provide nitrogen, vitamins, carbon, amino acids, sulphur and other essential nutrients.

Why Mueller-Hinton agar is used in AST?

Mueller-Hinton agar is the best medium for routine antibiotic susceptibility testing (AST) because of the following reasons: It shows acceptable batch-to-batch reproducibility for susceptibility testing. It supports satisfactory growth of most nonfastidious pathogens.

What pH is the Mueller-Hinton agar?

7.3 ± 0.1
Check prepared Mueller Hinton Agar to ensure the final pH is 7.3 ± 0.1 at 25°C. Dehydrated Appearance: Powder is homogeneous, free flowing, and beige. Prepared Appearance: Prepared medium is hazy and light to medium yellow.

Why is Mueller Hinton agar used instead of nutrient agar?

It is a loose agar. This allows for better diffusion of the antibiotics than most other plates. A better diffusion leads to a truer zone of inhibition. MHA shows acceptable batch-to-batch reproducibility for susceptibility testing.

What pH is the Mueller Hinton agar?

What is the use of Mueller Hinton II broth cation adjusted?

Mueller Hinton II Broth Cation-Adjusted may be used for inoculum preparation for MIC tests and for preparation of antimicrobial dilutions for the microdilution or macrodilution procedure. Details for the preparation of antimicrobial agents are provided in reference 1.

How are antimicrobial agents prepared in Mueller Hinton II?

Antimicrobial agents are prepared in serial 2-fold dilutions in Mueller Hinton II Broth and are inoculated with the test culture to give a final concentration of 5 x 105 CFU/mL. Following incubation at 35 °C, the presence of turbidity indicates growth of the organism.

Which organisms do not grow in Mueller Hinton II broth?

Microorganisms that require thymine or thymidine may be encountered in clinical specimens.14 These organisms may not grow in Mueller Hinton II Broth which is formulated with low levels of thymine and thymidine. Fastidious organisms such as Haemophilus, Neisseria and certain streptococci also will not grow or will grow poorly in this medium.

What is Mueller broth 2 used for in microbiology?

Mueller Hinton Broth 2 has been used for culturing Escherichia coli, Bacillus subtilis, Staphylococcus aureus and in the serial dilution of doxycycline. Suspend 22 g in 1000 ml distilled water. Heat if necessary to dissolve the medium completely.