Is AlphaLISA a fluorescence?

AlphaLISA and AlphaScreen No-wash Assays Binding of molecules captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent/fluorescent signal.

How does AlphaScreen work?

AlphaScreen relies on the use of “Donor” and “Acceptor” beads coated with a layer of hydrogel providing functional groups for bioconjugation. When a biological interaction between molecules brings the beads into proximity, a cascade of chemical reactions is initiated to produce a greatly amplified signal.

What is alfascreen?

Introduction. AlphaScreen is a bead-based, non-radioactive Amplified Luminescent Proximity Homogeneous Assay. When a biological interaction brings the beads together, a cascade of chemical reactions acts to produce a greatly amplified signal.

What is AlphaLISA used for?

AlphaLISA assays are versatile and can be used to detect analytes that are secreted, intracellular or membrane-bound. In addition to providing high-quality data and robust performance, AlphaLISA assays are simple and quick to optimize.

How does AlphaLISA assay work?

AlphaLISA Assay Principle​ AlphaLISA is a bead-based, no-wash luminescent technology. When Alpha Donor and Acceptor beads are brought together through binding to the target, a cascade of chemical reactions is set in motion, creating an amplified signal.

What is an HTRF assay?

HTRF (Homogeneous Time Resolved Fluorescence) is the most frequently used generic assay technology to measure analytes in a homogenous format, which is the ideal platform used for drug target studies in high-throughput screening (HTS).

What is HTRF assay?

What is fluorescence polarization assay?

Fluorescence polarization immunoassay (FPIA) has been the first applied in biochemistry. It is a competitive biochemical assay used to detect antigens or antibodies. In a fluorescence polarization immunoassay, an antigen bound to a fluorophore is used as tracer.

What is multiplex immunoassay?

Multiplex assays are a type of immunoassay capable of simultaneous measurement of multiple analytes. Multiplex assays are derived from the ELISA format, but instead of producing a single signal measurement, they produce multiple signal measurements.

Is Htrf the same as TR fret?

HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor.

Which of the following is an advantage of Htrf process?

The advantages of HTRF screens are that these screens are fast, inexpensive compared to many screens, specific, and have a robust fluorescent signal (1).