How much volume can a 6 well plate hold?

Growth Area of Tissue Culture Plates and Dishes

Growth Area (cm2) Media Volume (ml)
245 mm (square) 500 100-150
Dishes
6 well 9.5 1.9-2.0
12 well 3.8 0.76-1.14

How big is a 6 well plate?

6 Well Macroplates Manufactured from crystal clear and heat-resistant polystyrene for optimal analysis by microscopy With 35 mm diameter per well, it is a practical and space-saving alternative to six standard petri dishes Numeric coding allows easier identification of the wells Volume/well: 16 ml With…

How much media can 96-well plate?

For any cell type you can use a minimum of 100ul to a maximum of 200ul culture medium per well in a 96-well plate.

How much does it cost to seed a 6 well plate?

2D cultures

Culture plate format Number of viable cells per well/flask Volume per well/flask
6-well plate 2 x 106 2 ml
12-well plate 0.8 x 106 1 ml
24-well plate 0.48 x 106 0.5 ml
48-well plate 0.16 x 106 0.2 ml

How many cells are in a 96-well plate?

I haven’t worked specifically with those cells, but similar cells I’ve used are typically confluent in a 96-well at around 80-100K cells. It’s important to empirically determine in your hands the doubling rate and if this is the same at low vs.

How much protein is in a 6-well plate?

80% confluency (35mm / 3.5cm) of a 6-well plate contains ~1e6 (1 million) cells / up to 300 µg cytoplasmic protein.

How much volume does a 96-well plate have?

CulturPlate Microplates

Well format 24-well 96-well
#columns 6 12
Well volume 2.39 mL 400 µL
Recommended working volume 0.5-2.39 mL 80 µL-350 µL
Height (mm) 18.70 14.60

How much volume can a 96 well plate hold per well?

CulturPlate Microplates

Well format 24-well 96-well
Well volume 2.39 mL 400 µL
Recommended working volume 0.5-2.39 mL 80 µL-350 µL
Height (mm) 18.70 14.60
Length (mm) 127.80 127.76

How many cells does a 6-well plate have?

Useful information for various sizes of cell culture dishes and flasks

Catalog No. Cells at confluency*
Dishes
6-well 140675 1.2 x 106
12-well 150628 0.5 x 106
24-well 142475 0.24 x 106

How many cells are in a 96-well?

Typically, 20,000 – 75,000 cells are seeded per well in Oris™ and Oris™ Pro 96-well plates, and 2,500 – 10,000 per well in Oris™ Pro 384-well plates. For your first experiment, we recommend testing several different cell seeding densities as described in Appendix 1 in the assay protocol.

How much Ripa do I need for 6-well?

200-400 µl
Available Options

Plate Size / Surface Area Recommended Buffer Volume
100 mm2 500-1000 µl
6-well cell culture plate 200-400 µl per well
24-well cell culture plate 100-200 µl per well
96-well cell culture plate 50-100 µl per well

How much lysis buffer should I add to a 6-well plate?

Generally, 0.3-0.5 ×106 cells/3ml is recommended in each well of 6- well plates.