How much RNA is in a Bioanalyzer?

RNA must be submitted in 0.2mL tubes at no greater than 5µL volume. Total RNA in each sample must be between 200-500pg/µL and mRNA concentration must be between 500-5000pg/µL. If your sample is more concentrated please dilute with RNAse free water.

How does Bioanalyzer work RNA?

How does the Bioanalyzer work? The bioanalyzer instrument detects biomolecules by laser-induced fluorescence. Each LabChip® contains an interconnected set of microchannels that is used for separation of nucleic acid fragments based on their size as they are driven through it electrophoretically.

How long does a Bioanalyzer run take?

With each new kit, DNA ladder and gel aliquots must be prepared, which adds time to the first use. However, once this has been done, the instrument and chip take just approximately 15 minutes to prepare and 30 minutes to run, for up to 12 samples. In order to run a chip, the user must first prepare the gel-dye mix.

How much does a Bioanalyzer cost?

This model sells new from Agilent for $30,000. Comes with:Agilent 2100 G2938C Bi… Agilent 2100 Model G2938C Bioanalyzer Complete system-NGS Analysis!

What is Bioanalyzer used for?

Applications. The Bioanalyzer can be used to assess the size and quality of DNA over a range of sizes and concentrations. The DNA 1000 assay can be used with extracted, clean DNA to look at the size profile of a sample from 25bp to 1000bp at a concentration of 0.1-50ng/ul.

How do you load Bioanalyzer?

Adjust the chip selector: 1 Open the lid of the bioanalyzer and make sure that the electrode cartridge is inserted in the instrument. If not, open the latch, remove the pressure cartridge and insert the electrode cartridge. 2 Remove any remaining chip and adjust the chip selector to position (1).

How do you clean Bioanalyzer electrodes?

Clean the electrode pinset between each run with a dedicated cleaning chip containing 350 µL of RnaseZAP/water (refer to protocol). Empty the cleaning chip in between each run and replace with fresh liquid. Perform a thorough cleaning every 3 months or sooner if the electrode is suspected to be dirty.

How much does Bioanalyzer 2100 cost?

We have an excellentconditonAgilent 2100 G2938Ccomplete bioanalyzer system from Agilent Technologiesavailable for sale. This model sells new from Agilent for $30,000.

What does a Bioanalyzer do?

The Bioanalyzer is a chip-based capillary electrophoresis machine to analyse RNA, DNA, and protein. It is produced by Agilent and widely used, among other things, in RNA quality control measurements before downstream experiments like microarrays.