How is translation initiated in eukaryotes?

Translation initiation is a complex process in which initiator tRNA, 40S, and 60S ribosomal subunits are assembled by eukaryotic initiation factors (eIFs) into an 80S ribosome at the initiation codon of mRNA.

Which of the following protein interacts with the eIF4F for recruiting the pre initiation complex to the mRNA?

eIF4F complex formation. The interaction between eIF4G and eIF3 bridges the 40S ribosome and the mRNA, while eIF4G’s interaction with PABP is important for circularization of the mRNA.

What does the Kozak sequence do?

The Kozak consensus sequence (Kozak consensus or Kozak sequence) is a nucleic acid motif that functions as the protein translation initiation site in most eukaryotic mRNA transcripts.

What is the role of eif4 in the pre initiation complex?

It acts as an anchor, as well as a critical co-factor for eIF4A. It is also a substrate of S6K, and when phosphorylated, it promotes the formation of the pre-initiation complex. In vertebrates, eIF4H is an additional initiation factor with similar function to eIF4B.

What is Cap dependent translation?

Cap-dependent translation is initiated by the binding of the factor eIF4E to the cap domain of mRNA. Detailed x-ray crystal and NMR structures are available for eIF4E in association with cap-analogs, as well as domains of other initiation factors.

What is a poly A signal sequence?

Poly(A) signals consist of short redundant sequences that are not restricted to mapped gene 3′ ends. This is particularly apparent in the yeast Saccharomyces cerevisiae, in which the poly(A) signals are made of poorly conserved sequence motifs flanking the cleavage site (28).

What is a poly A tract?

poly(dT) tracts (poly(A) tracts), regions of DNA containing at least 20 contiguous dA residues on one strand and dT residues on the complementary strand, are found in about 2 X 10(4) copies interspersed throughout the human genome.