How is QBC test performed?
How is QBC test performed?
Method. 55-110µl of blood is taken in a QBC Malaria Tube which is internally coated with acridine orange and centrifuged for five minutes. When viewed under a fluorescent microscope, the malaria parasites are seen concentrated, shining in the background of dark Red Blood Cells.
What are the main diagnostic methods for malaria?
PCR is most useful for confirming the species of malarial parasite after the diagnosis has been established by either smear microscopy or RDT. Serology detects antibodies against malaria parasites, using either indirect immunofluorescence (IFA) or enzyme-linked immunosorbent assay (ELISA).
What is QBC test?
The QBC Malaria Test is a fluorescence microscopy-based malaria diagnostic test embraced by users around the world. More sensitive than Giemsa thick films, it can detect as little as one parasite per μL of blood and establish diagnosis earlier than thick film in 47% of low parasitemia (<10 parasites per μL) cases.
Which stain is used to identify malaria parasite in QBC test?
The quantitative buffy coat (QBC) method is used to identify malaria parasites in peripheral blood by staining the DNA of parasites with acridine orange.
What is QBC in parasitology?
Quantitative buffy coat (QBC) analysis, which is based on principle of centrifugal stratification of blood components, is a well-known and a very sensitive technique which can be used for the detection of malarial parasites in peripheral blood.
How is malaria diagnosed in the laboratory?
A laboratory professional will examine the slide under a microscope and look for parasites. Rapid diagnostic test. This test looks for proteins known as antigens, which are released by malaria parasites. It can provide faster results than a blood smear, but a blood smear is usually needed to confirm a diagnosis.
How does PCR detect malaria?
PCR is an alternative method of malaria diagnosis that allows for sensitive and specific detection of Plasmodium species DNA from peripheral blood. PCR may be more sensitive than conventional microscopy in very low parasitemias, and is more specific for species identification.
What is malaria QBC?
The quantitative buffy coat (QBC) technique is a method of diagnosing malarial parasites based on micro-centrifugation, fluorescence, and density gradient of infected red blood cells. The aim of the present study was to modify the QBC technique in order to reduce the cost per test of malaria diagnosis.
What is malaria PCR?
