How does the DNA fragment can joined together?

If two DNA molecules have matching ends, they can be joined by the enzyme DNA ligase. DNA ligase seals the gap between the molecules, forming a single piece of DNA.

How fragments of DNA can be separated?

Gel electrophoresis is a technique used to separate DNA fragments according to their size. DNA samples are loaded into wells (indentations) at one end of a gel, and an electric current is applied to pull them through the gel. DNA fragments are negatively charged, so they move towards the positive electrode.

How the DNA is separated and isolated?

DNA is isolated and extracted out of the cell by centrifugation. They are amplified through the polymerase chain reaction and multiple copies of DNA are produced. By the application of restriction enzymes, DNA is cut into fragments. Gel electrophoresis separates the DNA fragment based on their sizes.

What is ligation in DNA replication?

In molecular biology, ligation refers to the joining of two DNA fragments through the formation of a phosphodiester bond. An enzyme known as a ligase catalyzes the ligation reaction. In the cell, ligases repair single and double strand breaks that occur during DNA replication.

Which enzyme is used to bind DNA fragments together?

DNA Polymerase is the enzyme used to bind DNA fragments together.

Which enzyme is used to join nicks in the DNA strand?

Ligases
Ligases are versatile and ubiquitous enzymes that join the 3′ hydroxyl and 5′ phosphate ends to form a phosphodiester bond, making them essential in nicked DNA repair, and ultimately genome fidelity.

Which is the technique is used separation of large DNA fragments?

Agarose gel electrophoresis
Agarose gel electrophoresis is the technique which is used for the separation of the DNA fragments. This is the method which allows the separation of the DNA fragments on the basis of their size when there is an external supply of electric charge.

How can you separate DNA?

Gel electrophoresis is a laboratory method used to separate mixtures of DNA, RNA, or proteins according to molecular size. In gel electrophoresis, the molecules to be separated are pushed by an electrical field through a gel that contains small pores.

How are the DNA fragments separated by gel electrophoresis visualized?

The separated DNA fragments are visualized only after staining the DNA with the help of ethidium bromide followed by the exposure to UV radiation. The bright orange colour bands are shown.

Which of the following is known as joining enzyme of DNA?

DNA ligase is a specific type of enzyme, a ligase, (EC 6.5. 1.1) that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond.

Which enzyme joins replicated DNA fragments together during DNA replication?

The central enzyme involved is DNA polymerase, which catalyzes the joining of deoxyribonucleoside 5′-triphosphates (dNTPs) to form the growing DNA chain.