How does STZ induce diabetes?

Diabetes is induced by streptozotocin (STZ), a glucosamine–nitrosourea compound derived from Streptomyces achromogenes that is used clinically as a chemotherapeutic agent in the treatment of pancreatic β cell carcinoma. STZ damages pancreatic β cells, resulting in hypoinsulinemia and hyperglycemia.

What type of diabetes does STZ induce?

Type 1 diabetes can be induced in rodents by a single STZ injection,9,10 while type 2 diabetes can be induced by at least three approaches, which include STZ injection after administration of nicotinamide,11,12 high fat diet (HFD) feeding followed by a low-dose STZ injection,13 and STZ injection during the neonatal …

How does streptozotocin induce diabetes in mice?

Alternate Protocol : INDUCTION OF TYPE 1 DIABETES MELLITUS IN MICE USING A SINGLE, HIGH DOSE OF STREPTOZOTOCIN. A single, high dose of STZ (200 mg/kg) is directly toxic to pancreatic β-cells, rapidly causing diabetes, with blood glucose concentrations of >500 mg/dl within 48 hr (Like & Rossini, 1976).

How do you make a STZ solution?

Streptozotocin Injections For 100 ml, combine 1.05 g citric acid and 1.48 g sodium citrate in dH20. Ensure pH is 4.5, then bring up to 100 mL with water.

What are STZ mice?

Abstract. Streptozotocin (STZ) is an antibiotic that produces pancreatic islet β-cell destruction and is widely used experimentally to produce a model of type 1 diabetes mellitus (T1DM). Detailed in this unit are protocols for producing STZ-induced insulin deficiency and hyperglycemia in mice and rats.

Can STZ induced type 2 diabetes?

Administration of low-dose streptozotocin (STZ) causes β-cell dysfunction. Combining a HFD with low-dose STZ induces type-2 diabetes (T2D). HFD-STZ-T2D exhibits the metabolic characteristics similar to those seen in human T2D.

What is the meaning of STZ?

Streptozotocin or streptozocin (INN, USP) (STZ) is a naturally occurring alkylating antineoplastic agent that is particularly toxic to the insulin-producing beta cells of the pancreas in mammals.

How do you induce type 2 diabetes in mice?

To induce diabetes, animals of both sexes received daily intraperitoneal (i.p.) injection of STZ for 5 consecutive days at 55 mg/kg BW (a dose that is known to induce diabetes in male mice) or for females, 75 mg/kg BW of STZ.

What is db mouse?

db/db Mice (JAX® Mice Strain) Details. db/db mice are used to model phase 1 to 3 of diabetes type II and obesity. Mice that are homozygous for the diabetes spontaneous mutation (Leprdb) demonstrate morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and come to be hypoinsulinemic.