How does fluorescein detect blood?

Fluorescein reacts to the proteins and iron ions found in the hemoglobin and will fluoresce when exposed/excited to light at the 420nm to 485nm wavelengths peaking at about 460 to 480 nm range (blue light, near Ultraviolet).

Is fluorescein used to detect blood?

Fluorescein is a presumptive blood test for latent bloodstain detection. It has been used in forensic applications to reveal trace amounts of blood. Traces of latent blood can be detected even after repeated cleansing of the crime scene.

Is luminol or fluorescein better for blood detection?

A presumptive reagent for dilute blood detection other than luminol is fluorescein. The sensitivity of fluorescein approaches the sensitivity of detection levels of luminol. The fluorescein detection method offers the advantages of working in a lighted environment, and the reaction persists longer than luminol.

What methods are used to detect blood?

Further tests at the crime lab can determine if it is human blood or not. The Kastle-Meyer tests uses phenolphthalein and hydrogen peroxide, while HemaStix is a paper strip coated with TMB. This chemical is used to locate traces of blood, even if it has been cleaned or removed.

What is fluorescein test?

This is a test that uses orange dye (fluorescein) and a blue light to detect foreign bodies in the eye. This test can also detect damage to the cornea. The cornea is the outer surface of the eye.

What is luminol and fluorescein?

Luminol and fluorescein are common methods of latent blood detection at a crime scene. However, each method has limitations. Luminol requires total darkness to visualize latent blood, and the period of chemiluminescence (i.e. when compounds illuminate due to a chemical reaction) is relatively short.

What is a confirmatory blood test?

Confirmatory Blood Tests. If a stain is positive for the possibility of blood by a presumptive test, it must finally be subjected to a confirmatory test, or the possibility of a false positive remains. Confirmatory tests must be able to identify a substance with the lowest possible chances of a false positive.

What can be used instead of luminol?

In this thesis I critically evaluated three new luminol based formulas, one commonly used luminol formula and an alternative to luminol, fluorescein.

What is fluorescein used for?

Fluorescein is used to help in the diagnosis of a number of eye problems. When applied as a drop or within a strip of paper to the surface of the eye it is used to help detect eye injuries such as foreign bodies and corneal abrasions.

What does a positive fluorescein stain indicate?

Fluorescein staining indicates increased epithelial permeability of the cornea or conjunctiva by staining devitalized areas of the ocular surface.

What type of drug is fluorescein?

Organic compound
Fluoresceins
Fluorescein/Classification