How do you prepare a sample for FTIR?

Add about 1 to 2 % of your sample, mix and grind to a fine powder. For very hard samples, add the sample first, grind, add KBr and then grind again. The sample must be very finely ground as in the Nujol mulling technique to reduce scattering losses and absorption band distortions.

How do you prepare a sample for ICP-OES?

Heating acids is performed on a hot plate, or by using a microwave digestion system that employs pressurized vessels to produce even higher reaction temperatures. Acid digestion of soils and sludges is one example of solid sample preparation for ICP-OES.

How do you prepare a liquid sample for IR spectroscopy?

To prepare a liquid sample to IR analysis, firstly place a drop of the liquid on the face of a highly polished salt plate (such as NaCl, AgCl or KBr), then place a second plate on top of the first plate so as to spread the liquid in a thin layer between the plates, and clamps the plates together.

Why do you need to perform calibration before sample analysis using FTIR?

Fourier transform infrared (FTIR) spectrometers do the same job much more quickly by sending many wavelengths of light through the sample simultaneously. To do accurate, quantitative measurements, a spectrometer needs to be calibrated.

Why do we use potassium bromide in FTIR?

KBr is used as a carrier for the sample in IR spectrum and it is optically transparent for the light in the range of IR measurement. So that no interference in absorbence would occur. KBr, has a transmittance of 100 % in the range of wave number (4000-400 cm-1).

Which solvent is best used in infrared spectroscopy?

The most common solvents are Carbon Tetrachloride (CCl4) and Carbon Disulfide (CS2). Chloroform, methylene chloride, acetonitrile and acetone are useful solvents for polar materials.

Why KBr is used in IR spectroscopy sample preparation?

Why is water not used as solvent in IR?

Polar solvents such as water and alcohols are seldom used because they absorb strongly in the mid IR range and react with alkali-metal halides, such as NaCl, commonly used for cell windows.

Why do we use polystyrene film for FTIR calibration?

Polystyrene film is useful for routine checking of FT-IR spectrometer performance, but variations in film thickness and scattering can cause large variations in the results. Several pharmacopeias propose two tests for the resolution of Fourier transform infrared (FT-IR) spectrometers using films of polystyrene.

What is used for FTIR calibration?

Standard operating procedure to calibrate the Fourier Transforms Infra Red Spectrometer using polystyrene film.