How do I make a NP-40 lysis buffer?

1. Prepare 1% NP40 lysis buffer (LB). (To make 50 ml of 1% NP40 lysis buffer, add 5 ml of 10% NP40, 500 μl of 1 mM EDTA, 1.5 ml of 150 mM NaCl, and 1 ml of 20 mM Tris–Cl, pH 7.4; and bring the total volume to 50 ml using dH2O.) 2.

What is NP-40 substitute?

Applications Nonidet-P40 Br Substitute (NP-40 alternative) is used as nonionic surface-active agent. It is an effective emulsifier for solvents such as xylene. Furthermore in Biochemistry, Nonidet-P40 substitute (br) is a nonionic surfactant used in the isolation of membrane complexes.

What does NP-40 do in lysis buffer?

NP-40 (Nonidet P-40) and Triton X-100 are milder, nonionic detergents. They are good at solubilizing membrane proteins and for isolating cytoplasmic proteins. Proteins retain their native state in the presence of these detergents and protein-protein interactions can be preserved. These buffers can be used for co-IPs.

What is Nonidet P-40 Substitute?

Nonidet® P40 (substitute) is a non-ionic, non-denaturing detergent suitable for solubilizing and isolation of membrane proteins. Has been used to solubilize cerebral GABA receptors. Also useful in solubilization of proteins and lipids of the scallop gill ciliary membrane and of membranes from breast cancer tumors.

Is igepal the same as NP-40?

Nonidet P-40 and IGEPAL CA-630 are the same, those are merely different brand names. According to the manufacturer, Sigma-Aldrich, the two compounds are “chemically indistinguishable”. Both are octylphenoxy poly(ethyleneoxy)ethanol.

Does NP-40 denature proteins?

Sodium dodecyl sulfate is an ionic detergent that is strong enough to solubilize proteins and denature them. A weaker detergent such as NP-40 can be used to solubilize proteins but not denature them.

Is NP-40 same as igepal?

Nonidet P-40 and IGEPAL CA-630 are the same, those are merely different brand names. According to the manufacturer, Sigma-Aldrich, the two compounds are “chemically indistinguishable”.

How do you dilute NP-40?

Directions: 1) Mix 20 ml of NP-40 with 80 ml of ddH2O by stirring. 2) Store at 4°C. Note: Nonidet P-40 is chemically identical to Igepal-CA-630.

Is NP-40 a buffer?

NP40 Cell Lysis Buffer is suitable for the preparation of cell extracts to be analyzed by Antibody Bead Immunoassay (Luminex), ELISA, and Western blotting. It can also be used as a wash buffer for immunoprecipitation reactions. It is soluble in water.

Is igepal the same as NP 40?

How do you dilute NP 40?

What does NP-40 stand for?

NP-40 is an ethoxylated nonylphenol for non-ionic surfactants and can act as emulsifier and demulsifier agent. NP-40. Names. Other names. Polyethylene glycol nonylphenyl ether; Nonyl phenoxypolyethoxylethanol; Nonoxynol-40.