How chromatin is Remodelled during DNA repair of UV induced DNA damage in Saccharomyces cerevisiae?

We conclude that the global genome repair complex in yeast regulates UV-induced histone H3 acetylation by controlling the accessibility of the histone acetyl transferase Gcn5 in chromatin. The resultant changes in histone H3 acetylation promote chromatin remodelling necessary for efficient repair of DNA damage.

What happens to DNA with double-strand breaks?

And, if not repaired correctly, DSBs can cause deletions, translocations, and fusions in the DNA. These consequences are collectively referred to as genomic rearrangements, and they are commonly found in cancerous cells (Figure 1) (Aplan 2006).

What happens during chromatin remodeling in gene regulation?

Chromatin remodeling plays a central role in the regulation of gene expression by providing the transcription machinery with dynamic access to an otherwise tightly packaged genome.

What happens when chromatin is damaged?

These damaging agents can induce a wide variety of lesions in the DNA, such as double strand breaks, single strand breaks, oxidative lesions and pyrimidine dimers.

What is chromatin remodeling quizlet?

chromatin remodeling. he dynamic modification of chromatin architecture to allow access of condensed genomic DNA to the regulatory transcription machinery proteins, and thereby control gene expression.

What do chromatin remodelers do?

Chromatin remodelers are versatile tools that catalyze broad range of chromatin changing reactions including sliding of an octamer across the DNA (nucleosome sliding), changing the conformation of nucleosomal DNA and altering the composition of the octamers (histone variant exchange).

How are double stranded breaks in DNA repair?

Double-strand DNA breaks are common events in eukaryotic cells, and there are two major pathways for repairing them: homologous recombination (HR) and nonhomologous DNA end joining (NHEJ).

How do chromatin remodelers change the structure of the chromatin?

Chromatin remodelers have two types of effects on chromatin dynamics: 1) they package genomic DNA, and incorporate histones into the nucleosome, or 2) release DNA from the histones. After DNA replication, chromatin remodelers pack genomic DNA into nucleosomes.

What is chromatin material and how does it change?

The DNA + histone = chromatin As the cell initiates divisions by either meiosis or mitosis. During the interfaces, DNA is combined with the proteins and organized into a structure called chromatin. This chromatin is a thread like structure which condenses to form chromosomes just before the cell division occurs.