How are rabbit monoclonal antibodies made?

Rabbits are immunized and the resulting spleen cells are fused with partner cells to make an immortal cell line that expresses antibodies. The antibodies are derived from a single clone and characterized for performance in applications. The best clone is then selected for antibody production.

How do you purify an antibody?

For commercial scale operations, IgM antibodies are usually purified by a combination of techniques, including ammonium sulfate precipitation followed by gel filtration, ion exchange chromatography or zone electrophoresis.

Why are rabbit antibodies used?

The useful qualities of using rabbit monoclonal antibodies are attributed to the rabbit’s immune system, which is able to generate a larger range of high affinity antibodies, compared to mice. This makes the chance of finding immunoglobulins that are able to function in a range of applications far more likely.

Why do we purify antibodies?

We use the specificity of the antigen/antibody binding for immunoprecipitation and ELISA assays. We use flurophore-conjugated or enzyme-tagged antibodies for labeling molecular targets on individual cells and whole tissue. We use antibody purification to obtain antibodies for biosensors to detect disease.

Why is immunoaffinity chromatography used?

Immunoaffinity chromatography (IAC) combines the use of LC with the specific binding of antibodies or related agents. The resulting method can be used in assays for a particular target or for purification and concentration of analytes prior to further examination by another technique.

What is immunoaffinity capture?

Immunoaffinity chromatography is a method for separating target antibodies or antigens from a heterogenous solution. It is column-based, which means that the solution is flowed through a column and eluted at the other end. The column is pre-functionalized with the capture antibody or antigen.

What is the rabbit monoclonal antibody development process?

Our comprehensive rabbit monoclonal antibody development process includes peptide or protein antigen design, rabbit immunization, phage library creation, panning for lead identification, cloning of lead sequences into proprietary mammalian expression vectors, and antibody expression.

Do rabbit monoclonal antibodies (mAbs) exhibit high levels of citrus mosaic virus?

Although rabbit monoclonal antibodies (mAbs) exhibit high speci … Citrus mosaic virus (CiMV) is one of the causal viruses of citrus mosaic disease in satsuma mandarins (Citrus unshiu). Prompt detection of trees infected with citrus mosaic disease is important for preventing the spread of this disease.

What is a novel Rabbit immunospot array assay on a chip?

A novel rabbit immunospot array assay on a chip allows for the rapid generation of rabbit monoclonal antibodies with high affinity. PLoS ONE2012; 7: e52383.

Can rabbit monoclonal antibodies be used to detect cytomegalovirus (cimv)?

Although rabbit monoclonal antibodies (mAbs) exhibit high specificity and affinity, their applicability is limited by technical difficulties associated with the hybridoma-based technology used for raising these mAbs. Here, we demonstrate a feasible CiMV detection system using a specific rabbit mAb against CiMV coat protein.