Do neutrophils express Ly6G?

Neutrophil-mediated release of granules and chromatin generates neutrophil extracellular traps that sequester and kill extracellular microbes (6–8). Neutrophils also secrete chemokines and recruit additional immune cells (9). In mice, circulating neutrophils specifically express the Ly6G cell surface protein (10).

Do macrophages express Ly6G?

Flow cytometry and fluorescence-activated cell sorting (FACS) analysis demonstrated that Ly6G/Ly6C markers are superior to Gr-1 for identifying splenic neutrophils, eosinophils, and subsets of monocytes/macrophages.

Do T cells express Ly6G?

Later, several other types of inflammatory/immunological cells, including DCs and CD8+ T cells [19, 20], were shown to express this molecule. The anti-Gr-1 antibody recognizes the Ly6 superfamily (glycosylphosphatidylinositol-anchored membrane glycoproteins), which includes Ly6G and Ly6C.

What is ter119?

TER-119 is a lineage marker for erythroid cells from early proerythroblast to mature erythrocyte stages in adult blood, spleen, and bone marrow. It is also present in yolk sac, and fetal and newborn liver.

What is Ly6G a marker for?

Ly6G is a good marker for detection of peripheral neutrophils, monocytes and granulocytes. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation.

What is the function of Ly6G?

Ly6G is a glycosylphosphatidylinositol (GPI)–anchored protein of unknown function that is commonly targeted to induce experimental neutrophil depletion in mice.

What is Ly6G?

Ly6G (Lymphocyte antigen 6 complex locus G6D) is a component of the myeloid differentiation antigen Gr-1, together with Ly6C. The Ly6G protein has a molecular weight of approximately 21-25 kDa and is expressed by myeloid derived cells in the bone marrow.

Do eosinophils express Ly6G?

While Balb/c eosinophils predominantly express Ly6G, eosinophils from C57Bl/6 mice preferentially express Ly6C.

What are neutrophil markers?

Neutrophils are granule-containing, polymorphonuclear leukocytes that develop in the bone marrow from myeloid precursors.

What cells are CD45+?

CD45 (lymphocyte common antigen) is a receptor-linked protein tyrosine phosphatase that is expressed on all leucocytes, and which plays a crucial role in the function of these cells.