How do you design a TaqMan probe?
How do you design a TaqMan probe? TaqMan® design parameters used by Beacon Designer™ & AlleleID® Tm Criteria: The primer melting temperature (Tm) should be around 58-60 oC, and TaqMan® probe Tm should be 10 oC higher than the Primer Tm. Length Criteria: Primers should be 15-30 bases in length. GC Content: The G+C content […]
Does GFP antibody recognize mCherry?
Does GFP antibody recognize mCherry? Q: Does this antibody cross react with dsRed or mCherry? A: The anti-GFP antibody was made using the N-terminal of GFP as an antigen and will not react with RFP proteins dsRED or mCherry, since their sequences are so different. Can I use mCherry and GFP? Due to the spectral […]
Did Akhenaten have breasts?
Did Akhenaten have breasts? Akhenaten, a pharaoh during Egypt’s 18th Dynasty credited with starting the practice of worshipping one God, fathered six children. He was often portrayed in sculptures and carvings with a thin neck, elongated head, large buttocks, breasts, and even a prominent belly, suggesting pregnancy. Did Akhenaten have acromegaly? Surprisingly, in later images […]
Is Volvo shift automatic?
Is Volvo shift automatic? I-Shift is the result of many years of hard work by skilled engineers and feedback from customers. When I-Shift was first launched in 2001, Volvo Trucks confirmed its strong belief in a clutch-based transmission system as the most efficient design for automatic gear-changing. What kind of transmission does Volvo use? Geartronic […]
Can you get watery discharge after implantation?
Can you get watery discharge after implantation? After implantation, mucus tends to be thick, gummy, and clear in color. Some women experience implantation bleeding, or spotting. This can occur 6 to 12 days following conception. Unlike your normal period, implantation bleeding should stop after 24 to 48 hours. Is discharge sticky after implantation? Cervical mucus […]
What causes thermoregulation problems?
What causes thermoregulation problems? Various factors can contribute to this, including infections, extreme weather, medications, and other health conditions. Thermoregulation disorders can be a medical emergency. If a person has symptoms of hypo- or hyperthermia, it is important to dial 911 or the number of the nearest emergency department. What is Poikilothermia? Poikilothermia, the inability […]
What is 3 oz of chicken strips?
What is 3 oz of chicken strips? 3 ounces of chicken will be roughly the same size and width as a deck of standard playing cards. The palm of your hand. 3 ounces of cooked chicken is about the same size as the average palm. This measurement doesn’t include your fingers or thumbs. How many […]
What is the most popular food in Denver?
What is the most popular food in Denver? 10 Iconic Foods Every Denverite Should Try At Least Once Street Tacos. Jing/Flickr. Meats. The Fort Restaurant/Facebook. Rocky Mountain Oysters. Wally Gobetz/Flickr. Cheeseburgers. Ben D./Flickr. Green Chile Cheese Fries. Michael W./Yelp. Veggies. City, O’ City/Facebook. Denver Omelet. Stacy L./Yelp. Cinnamon Rolls. What’s the best place to eat […]
What is cell theory structure and function?
What is cell theory structure and function? Modern interpretation. The generally accepted parts of modern cell theory include: All known living things are made up of one or more cells. All living cells arise from pre-existing cells by division. The cell is the fundamental unit of structure and function in all living organisms. What is […]
Who got rich in Congress?
Who got rich in Congress? List of current members of the United States Congress by wealth Rank Name Net worth ($ million) 1 Dianne Feinstein 1098.63 2 Rick Scott 259.7 3 Mark Warner 214.1 4 Greg Gianforte 189.3 Why is the Senate called the millionaires club? Why was the Senate called the “Millionaires’ Club” in […]
What is The Wolverine Extended Cut?
What is The Wolverine Extended Cut? In this exclusive longer version with more brutally intense action, Hugh Jackman returns as The Wolverine and faces his ultimate nemesis in a battle that takes him to modern day Japan. How much longer is The Wolverine Extended Cut? This is a comparison between the Theatrical Version and the […]
Why is an action potential non decremental?
Why is an action potential non decremental? Action potentials travel in a non-decremental manner down the axon, with the voltage constantly being regenerated along the way, unlike graded potentials which quickly diminish over short distances. What are the two most commonly discussed ions when describing an action potential and how do they move to create […]