Do primers need to be phosphorylated?
Do primers need to be phosphorylated? No, there is no need to purchase phosphorylated primers nor to phosphorylate the PCR product. Any primer you already have in your lab can be used for cloning into pSpark® DNA Cloning Kits. Phosphorylated primers do not inhibit ligation and thus can also be used. Why do primers need […]
Who benefited from the railroads in the Civil War?
Who benefited from the railroads in the Civil War? Every major Civil War battle east of the Mississippi River took place within twenty miles of a rail line. Railroads provided fresh supplies of arms, men, equipment, horses, and medical supplies on a direct route to where armies were camped. Which side used railroads in the […]
Is Archaeognatha an insect?
Is Archaeognatha an insect? Archaeognatha are small insects with elongated bodies and backs that are arched, especially over the thorax. They have three long tail-like structures, of which the lateral two are cerci, while the medial filament, which is longest, is an epiproct. The antennae are flexible. How many species of thysanura are there? 370 […]
Why do most forex brokers not accept US clients?
Why do most forex brokers not accept US clients? The reason for this is quite simple – capital requirements. While a broker has to have around $100,000 – $500,000 of locked capital to obtain one of the European licenses, NFA requires quite an enormous amount of capital to be able to operate in the US […]
Why are my Ctrl shortcuts not working?
Why are my Ctrl shortcuts not working? Enable Sticky Keys Keyboard shortcuts could fail to work if you don’t press the key combinations at the same time. If you are having difficulties pressing multiple keys at the same time, you should enable Sticky Keys. This Windows 10 feature allows you to press the shortcut keys […]
Where is the greater trochanter?
Where is the greater trochanter? hip The greater trochanter is located at the top of the thighbone (femur) and is the most prominent and widest part of the hip. Is trochanter same as hip? A trochanter is a tubercle of the femur near its joint with the hip bone. In humans and most mammals, the […]
Why did Mark Whipple quit Pitt?
Why did Mark Whipple quit Pitt? No reason was given for Whipple’s decision to step down, but local radio host Andrew Fillipponi tweeted Tuesday afternoon that Whipple’s decision is “family related.” Source says Mark Whipple’s decision to step away at Pitt is family related. Wish him the best. How much does pat narduzzi make? Pitt […]
What is a normal ATP level?
What is a normal ATP level? 1 to 10 mmol/L Normally cellular ATP concentration is maintained in the range of 1 to 10 mmol/L, with a normal ratio of ATP/ADP of approximately 1000. Totally quantity of ATP in an adult is approximately 0.10 mol/L. What does a high ATP level mean? ATP, ADP, and NADH […]
What is Waukesha known for?
What is Waukesha known for? Waukesha is home to the great Les Paul who invented the solid body electric guitar, first 8-track tape recorder, and more. This is the reason why Waukesha is also coined “Guitar Town”. Waukesha is the first home to resident Indian tribes like Sauk, Menomonie, Winnebago and Potawatomi. Is Waukesha WI […]
What are non-essential carbohydrates?
What are non-essential carbohydrates? Except for dietary fiber, all dietary carbohydrates are considered nonessential. Every other major class of nutrients contains multiple essential compounds. For example, there are nine essential amino acids, at least two essential fatty acids, and many essential vitamins and minerals. What are non-essential nutritional requirements? Types of Nutrients Some building blocks […]
Does Harvard offer joint degrees?
Does Harvard offer joint degrees? As an MPP or MPA/ID student at Harvard Kennedy School, you can pursue a joint degree—either an MBA at Harvard Business School (HBS) or a JD at Harvard Law School (HLS)—that involves carefully crafted and integrated coursework. How much does a Harvard JD MBA cost? Your Student Budget How to […]
How do you dilute trypan blue?
How do you dilute trypan blue? Mix 1 part of 0.4% trypan blue and 1 part cell suspension (dilution of cells). Allow mixture to incubate appoximately 3 minutes at room temperature. Note: Cells should be counted within 3-5 minutes of mixing with trypan blue, as longer incubation periods will lead to cell death and reduced […]