What is VEGF Elisa?

The Human VEGF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate.

Is VEGF an antigen?

VEGF Protein Human Recombinant | VEGF-A Antigen | ProSpec.

How do you measure your VEGF?

Typically, VEGF in either serum or plasma samples from anti-VEGF–treated patients is measured with a sandwich enzyme-linked immunosorbent assay, with the R&D Systems kit (Minneapolis, MN, USA) being the most commonly used.

What is anti VEGF antibody?

Antibodies that detect VEGF can be used in several scientific applications, including Western Blot, ELISA, Immunohistochemistry (Paraffin), Immunocytochemistry and Immunohistochemistry (Frozen). These antibodies target VEGF in Human, Mouse, Rat, Canine and Bovine samples.

What is the role of VEGF?

VEGF’s normal function is to create new blood vessels during embryonic development, new blood vessels after injury, muscle following exercise, and new vessels (collateral circulation) to bypass blocked vessels. It can contribute to disease.

How is VEGF produced?

VEGF is produced by hypertrophic chondrocytes in the growth plate where it co-ordinates extracellular matrix (ECM) remodelling, angiogenesis, and bone formation. VEGF is expressed in the synovial fluid of patients with rheumatoid arthritis.

What is a normal VEGF level?

Plasma VEGF levels have a range of 37–310 pg ml−1 (cancer) vs 27–30 pg ml−1 (healthy). Tumour cytosol VEGF values range from 140 to 693 pg mg−1 protein, but there is no control equivalent in healthy subjects or other tissues in these studies.

Why is my VEGF high?

An elevated VEGF level in the serum may indicate the presence of cancer. Chronic states of hypoxia such as COPD may increase VEGF levels. In addition, people with serious or chronic wounds may have elevated VEGF levels. VEGF is produced in people with low platelet levels (thrombocytopenia).

What is an example of an anti-VEGF agent?

There are three main types of anti-VEGF drugs in use: aflibercept (EyeleaTM), bevacizumab (Avastin) and ranibizumab (LucentisTM). Only aflibercept and ranibizumab have received marketing authorisation for the treatment of DMO.

What does VEGF stand for?

A substance made by cells that stimulates new blood vessel formation. Also called vascular endothelial growth factor.

What is VEGF test?

This test measures the amount of vascular endothelial growth factor (VEGF) in your blood. VEGF is a substance that helps encourage the growth of new blood vessels. Your body makes more VEGF in certain cases.