Does Vibrio grow on blood agar?

Vibrio cholerae on blood agar cholerae frequently produces greenish clearing around areas of heavy growth but not around well-isolated colonies. This phenomenon, often describes as “hemodigestion,” is produced by metabolic by-products which are inhibited by anaerobic incubation of the blood agar plate.

What is the enrichment media for Vibrio species?

Alkaline peptone water (APW) remains the recommended enrichment medium for vibrios in parallel with either salt polymyxin broth (SPB) or glucose teepol (or sodium dodecylsulphate) salt broth (GTSB) when tests for V. parahaemolyticus are required.

Does Vibrio grow on MacConkey?

Most Vibrio species can grow on standard media, including blood and MacConkey agars. They are usually non-lactose fermenters, with the exception of V. vulnificus, which ferments lactose in 85% of cases (3).

Which medium are used to differentiate the colonies of Vibrio cholerae and V. parahaemolyticus?

thiosulfate citrate bile salts sucrose agar
In contrast, in smaller clinical laboratories or in laboratories investigating epidemics, thiosulfate citrate bile salts sucrose agar is best because it is commercially available and easy to prepare and can be used to distinguish colonies of suspect V. cholerae from V. parahaemolyticus.

What Gram stain is Vibrio cholerae?

The cholera vibrios are Gram-negative, slightly curved rods whose motility depends on a single polar flagellum.

Which of the following culture media would be most suitable for the isolation of Vibrio parahaemolyticus from fecal samples?

Several selective agar media have been devised, but thiosulfate citrate bile salts sucrose (TCBS) agar is recommended for the isolation of V. parahaemolyticus. In clinical microbiology laboratories, MacConkey agar containing 0.5% additional NaCl is also convenient for routine culture of diarrheal stools.

How do you culture and isolate Vibrio cholerae?

Isolation and identification of Vibrio cholerae serogroup O1 or O139 by culture of a stool specimen remains the gold standard for the laboratory diagnosis of cholera. Cary Blair media is ideal for transport, and the selective thiosulfate–citrate–bile salts agar (TCBS) is ideal for isolation and identification.

Is Vibrio cholera a lactose fermenter?

V. cholerae strains are usually non-fermenters or late fermenters of lactose. A deviation from this previously accepted finding may cause error in identification.

What makes TCBS agar selective?

TCBS agar is both selective and differential. It is highly selective for Vibrio species and differential due to the presence of sucrose and the dyes. Sucrose fermentation produces acid, which converts the colour of bromothymol blue or thymol blue.

What stain is used for cholera?

An effective selective medium is thiosulfate-citrate-bile salts-sucrose (TCBS) agar, on which the sucrose-fermenting cholera vibrios produce a distinctive yellow colony.

Is Vibrio Gram positive or negative?

gram-negative bacteria
Vibrio are gram-negative bacteria that are naturally found in warm, salty marine environments, such as salt water and brackish water. More than 20 Vibrio species can cause the human illness vibriosis.

What media is used to grow Vibrio vulnificus?

TCBS agar was originally developed for the isolation of Vibrio spp. that are pathogenic in humans. It has been widely recommended for the isolation of V. vulnificus from clinical samples, but it has also been frequently used as the primary isolation medium in ecological studies.