What is C18 column?

A C18 column is an example of a “reverse phase” column. Reverse phase columns are often used with more polar solvents such as water, methanol or acetonitrile. The stationary phase is a nonpolar hydrocarbon, whereas the mobile phase is a polar liquid. The same approach can also be used in TLC.

What is RP18 column?

Product Description. These reversed-phase columns contain Waters innovative embedded polar group technology that “shields” the silica residual silanol surface from highly basic analytes. The reduced silanol activity for the SymmetryShield RP18 column significantly improves peak shape and resolution.

What is ODS and BDS?

ODS and BDS are two columns used for reverse-phase chromatography. The key difference between ODS and BDS column is that ODS column contains free –OH functional groups, whereas BDS column contains deactivated –OH groups. Moreover, ODS columns have high peak tailing while BDS columns are designed to reduce peak tailing.

What is C18 silica gel?

C18 Silica Gel (Octadecyl Phase) C18 silica gel is used for the separation of nonpolar to moderately polar compounds such as: Fatty acids, Glycerides, Polycyclic aromatics, Esters (phthalates), Fat-Soluble vitamins, Steroids, Prostaglandins, PTH amino acids.

What is column end capping in HPLC?

Endcapping of the HPLC Column bonded phase minimizes silanol interaction and protects the silica support from dissolution. Different endcapping procedures are utilized today in modern HPLC column phases. Endcapping refers to the replacement of available silanol groups in a bonded stationary phase with TMS.

What is C8 and C18 column?

Definition. C8 column refers to a type of column used in the reverse-phase chromatography, containing octylsilane as its stationary phase, while the C18 column refers to another type of column used in the reverse-phase chromatography, containing octadecylsilance as the stationary phase.