What is V type pump?

Abstract. Vacuolar-type ATPase (V-ATPase), initially identified in yeast and plant vacuoles, pumps protons into the lumen of organelles coupled with ATP hydrolysis. The mammalian counterpart is found ubiquitously in endomembrane organelles and the plasma membrane of specialized cells such as osteoclasts.

What is V ATPase?

V-ATPases acidify a wide array of intracellular organelles and pump protons across the plasma membranes of numerous cell types. V-ATPases couple the energy of ATP hydrolysis to proton transport across intracellular and plasma membranes of eukaryotic cells.

How is V ATPase activity measured?

A bafilomycin A-sensitive ATPase activity is measured by detection of the released phosphate and the quench of acridine orange fluorescence due to H+ transport into the vesicles.

What is ATPase proton pump?

The vacuolar (V-)ATPases are ATP-driven proton pumps that function to acidify intracellular compartments and transport protons across the plasma membrane. They function in various normal and disease processes, including membrane trafficking, protein degradation, bone resorption and tumour metastasis.

What does the V type ATPase in the plant vacuole do?

Function of the V-ATPases Canonical functions: transportation of protons across membranes. Besides pumping protons to the extracellular space, PPs are in charge of acidifying lysosomes. This is an important and essential step for protein processing and degradation in endocytosis and autophagy.

What is an F type ATPase?

F-ATPase, also known as F-Type ATPase, is an ATPase/synthase found in bacterial plasma membranes, in mitochondrial inner membranes (in oxidative phosphorylation, where it is known as Complex V), and in chloroplast thylakoid membranes.

What is an F-type ATPase?

How many types of proton pumps are present in chloroplast?

There are three types of proton pumps: P-type, V-type, and F-type. The P-type proton pump has a cell membrane of eukaryotes and is characterized by transporting H+.

What is the role of ATPase in photosynthesis and cellular respiration?

Plasma membrane H+-ATPase, a crucial enzyme for plant life, acts as a primary transporter in fungi and plants. It actively transports H+ to extracellular spaces using the energy provided by ATP hydrolysis.