Why is BSA a good blocking agent?

Typically, when working with phosphorylated proteins, BSA tends to work better as a blocking agent. This is because milk has a variety of proteins, one being phosphoprotein casein, which leads to a higher background. Of course with all advice, there are special cases.

Does BSA bind to antibody?

When BSA is added to an immunoassay it binds to sites where there is potential for non-specific binding without interfering with antibody/antigen binding. An added bonus of using BSA is that it stabilizes proteins while in solution.

Why is BSA used during the blocking step of ELISA plate preparation assay?

The enzyme-linked immunosorbent assays (ELISA) is an extremely common and powerful laboratory technique for detecting proteins by antibodies. Researchers frequently use bovine serum albumin (BSA) as a blocking agent to prevent non-specific binding of antigens and antibodies to the microtiter well.

Why BSA is added in ELISA?

The enzyme-linked immunosorbent assay (ELISA) is an extremely common and powerful laboratory technique for detecting proteins by antibodies. Researchers frequently use bovine serum albumin (BSA) as a blocking agent to prevent non-specific binding of antigens and antibodies to the microtiter well.

Does IgG bind BSA?

The IgG and IgA antibody binding capacity of recombinant BSA correlates well with the antibody binding capacity of native BSA, irrespective of the probable loss of some conformational epitopes on recombinant BSA.

How do you remove BSA from antibodies?

BSA can be effectively separated when present at concentrations of up to 0.5%. If BSA is present at higher concentrations, dilute the antibody mix with de-ionized, distilled water until BSA concentration is 0.5% or less.

Why is 5sa BSA blocking solution in ELISA?

A blocking buffer is a solution of irrelevant protein, mixture of proteins, or other compound that passively adsorbs to all remaining binding surfaces of the plate. The blocking buffer is effective if it improves the sensitivity of an assay by reducing background interference and improving the signal-to-noise ratio.