What does Gcamp stand for?

Green fluorescent protein. Myosin light-chain kinase.

What is a genetically encoded calcium indicator?

The genetically encoded calcium indicator (GECI), which binds to calcium ions and emits fluorescence visualizing intracellular calcium concentration, enables detection of in vivo neuronal firing activity. Various GECIs have been developed and can be chosen for diverse purposes.

What is GECIs?

GECIs measure calcium, the key signal molecule underlying cell functions such as heart, vessel, and airway contraction, lung secretion, autonomic neurotransmission, and immunocyte function. GCaMP, originally developed by Junichi Nakai is an example of a GECI.

What is iGluSnFR?

iGluSnFR was developed in 2013 by Loren Looger’s lab at HHMI Janelia Research Campus to give researchers the ability to monitor glutamate release from neurons and other brain cells in vivo. Glutamate plays a variety of roles in synaptic communication and can trigger other forms of neuronal signaling and regulation.

What is 2 photon calcium imaging?

Two-photon calcium imaging is a powerful means for monitoring the activity of distinct neurons in brain tissue in vivo. In the mammalian brain, such imaging studies have been restricted largely to calcium recordings from neurons that were individually dye-loaded through microelectrodes.

Is calcium imaging used in humans?

Calcium imaging, also referred to as calcium mapping, is also used to perform research on myocardial tissue.

Is calcium imaging electrophysiology?

Calcium imaging using fluorescent protein sensors is a powerful method for recording activity in large neuronal populations[5,8]. In systems neuroscience, cellular calcium imaging fills a complementary role to extracellular electrophysiology.

What does Genpact stand for?

generating business impact
Genpact (NYSE: G) stands for “generating business impact.” We are a global leader in digitally-powered business process management and services.

When was calcium imaging invented?

Scientists at the University of California Berkley developed the first fluorescein-based calcium indicator in the 1980s. This breakthrough meant intracellular calcium could be visualised and quantified.