What is TFA used for?

TFA has important properties for its use in organic synthesis, such as low boiling point, high dielectric constant and strong acidity. A number of pharmaceutical intermediates and agrochemicals should be prepared using TFA as a reagent or its derivatives during their synthesis.

Is TFA a salt?

Peptides purified by HPLC are often in the form of a trifluoroacetate (TFA) salt, because trifluoroacetic acid is used as a solvent in reversed-phase HPLC separation.

Is trifluoroacetic acid a strong acid?

Trifluoroacetic acid is a very strong acid and is extensively used in organic chemistry. It provides a convenient way to introduce a trifluoromethyl group into a more complex organic molecule.

Is TFA soluble in hexane?

6Solubility. Miscible with ether, acetone, ethanol, benzene, carbon tetrachloride, hexane.

How corrosive is TFA?

* Trifluoroacetic Acid is a CORROSIVE CHEMICAL and contact can severely irritate and burn the skin and eyes with possible eye damage. * Breathing Trifluoroacetic Acid can irritate the nose, throat and lungs causing coughing, wheezing and/or shortness of breath.

Is TFA toxic?

As an acid or as a salt TFA is low to moderately toxic to a range of organisms. Based on current projections of future use of HCFCs and HFCs, the amount of TFA formed in the troposphere from substances regulated under the MP is too small to be a risk to the health of humans and environment.

Why TFA is used in HPLC?

TFA is widely used as a mobile phase additive in the HPLC separation of biological molecules, such as proteins and peptides, because it acts as an ion-pairing reagent and equilibrates quickly so that it can be used with gradient elution.

What is TFA solvent?

TFA is a versatile solvent for NMR spectroscopy (for materials stable in acid). It is also used as a calibrant in mass spectrometry. TFA is used to produce trifluoroacetate salts.

How do you neutralize TFA?

To remove traces of TFA you can use exsiccator with KOH and – optionally – some heat. If you have the salt with TFA you could dissolve your product in water add some NH3 – until you have slight alkalline conditions – and extract your product with CHCl3 or DCM, evaporate and dry over KOH.

How do I remove TFA from a reaction?

TFA can be removed using rotary evaporator. Add Methanol (5~6 times) and remove methanol by rotary evaporator. Most of the TFA is removed using this procedure and traces can be removed using vacuum.

Is TFA organic?

Trifluoroacetic acid (TFA) is an organofluorine compound, an organic acid with the formula CF3COOH.

How do I get rid of TFA?

TFA and HCl are both strong acids that will protonate any amino group. They can be removed by performing anion exchange on the same reversed phase HPLC on which the peptide was purified.