What is the V3 region of 16S rRNA?
What is the V3 region of 16S rRNA?
Table 2.
| Region | Number of fragments | Size (range) |
|---|---|---|
| V3 | 486,589 | 152.33 – 197.84 |
| V4 | 478,491 | 281.48 – 284.61 |
| V5 | 483,462 | 137.44 – 144.37 |
| V6 | 489,859 | 150.00 – 159.06 |
Why is the 16S rRNA a good target for sequencing?
A nearly complete 16S rRNA gene sequence is therefore very easy to obtain for a novel bacterial isolate, and it provides enough phylogenetic information to identify the isolate at least down to the genus level, thanks to the huge database of 16S rRNA gene sequence information that is publicly available and easily …
Why is V3 V4 region of 16S rRNA?
The main reason for using V3/V4 region in majority of the cases that these regions contain the maximum nucleotide heterogeneity and displays the maximum discriminatory power. However, it should be noted that no single region can differentiate among all bacteria.
What is V4 region in 16S?
In most microbial species, the 16S fourth hypervariable (V4) region is approximately 254 bp, and only deviates from this length by a few basepairs.
What is the function of 16S rRNA?
The 16S rRNA is the central structural component of the bacterial and archaeal 30S ribosomal subunit and is required for the initiation of protein synthesis and the stabilization of correct codon-anticodon pairing in the A site of the ribosome during mRNA translation [1].
Why is 16S rRNA used as an indicator of phylogeny?
Since 16S rRNA gene is conserved in bacteria, and contain hypervariable regions that can provide species-specific signature sequences, 16S rRNA sequencing is widely used in identification of bacteria and phylogenetic studies. 16S rRNA sequencing is featured by fast speed, cost-efficiency, and high-precision.
What is 16S rRNA analysis?
16S rRNA gene sequencing is commonly used for identification, classification and quantitation of microbes within complex biological mixtures such as environmental samples (ex marine water) and gut samples (ex human gut microbiome).
What does analysis of 16S rRNA sequences show?
In general, the comparison of the 16S rRNA gene sequences allows differentiation between organisms at the genus level across all major phyla of bacteria, in addition to classifying strains at multiple levels, including what we now call the species and subspecies level.
