How do you choose diluting fluid for WBC?

Dilutions should be based on the turbidity of the fluid or on the number of cells seen on the hemacytometer when using an undiluted sample. A WBC count of approximately 200/μL or an RBC count of approximately 400/μL3 causes a fluid to be slightly hazy.

What are the diluting fluids used for WBC count and RBC count?

The diluting fluid for RBCs is isotonic saline. Diluting fluids for WBCs include glacial acetic acid to lyse the RBCs, or Türk solution, which contains glacial acetic acid and methylene blue to stain the nuclei of the WBCs.

What is the other name of WBC dilution fluid?

In hemocytometry, Türk’s solution (or Türk’s fluid) is a hematological stain (either crystal violet or aqueous methylene blue) prepared in 99% acetic acid (glacial) and distilled water.

Is WBC diluting fluid hypertonic?

WBC diluting fluids: The leukocyte diluting fluid is a hypotonic solution which lakes the red cells but preserve the white cells.

Why RBC diluting fluid is used?

Used as diluting fluid for blood specimens to count RBCs in microscopy by hemocytometry. RBC diluting fluid is isotonic with blood, hence hemolysis does not take place. Normal Saline also can be used. But it causes slight creation of red blood cells and allows rouleaux formation.

What is hayem’s fluid?

Mercuric chloride – 0.25 gm. Sodium sulphate – 2.50 gm. Sodium chloride – 0.50 gm. Distilled water – 100.0ml.

What are the different RBC diluting fluids?

Dacie’s fluid, Gower fluid and Isotonic Saline are other diluting agents which can be used for dilution of blood for RBC count.

What is Turk’s solution used for?

Türk’s solution is a hematological stain (crystal violet or aqueous methylene blue) that destroys the membrane of red blood cells (RBCs), white blood cells (WBCs) and platelets and platelets within a blood sample, and stains the nuclei of the white blood cells, making them easier to see and count.

What is the most common WBC diluting fluid used in automation?

Three per cent [3%] acetic acid is the diluent of choice for a WBC. However, one percent hydrochloric acid or Turk’s diluting fluid may also be used. [Turk’s fluid contains gentian violet which stains the nuclei of wbcs, making them more visible].

What is the best RBC diluting fluid?

However, Hayem’s diluting fluid gives the better results. This is a special type of glass chamber that is used for the cell counting, especially for Blood cells.

What is WBC pipette?

WBC Pipette or White Blood Cell Pipette is used for mixing diluting fluid and blood. This pipette is used in higher dilution processes, such as higher total sperm count, platelet count, total leukocyte count, RBC count in severe anaemia, etc.