What does RecA do in homologous recombination?
What does RecA do in homologous recombination?
RecA’s association with DNA major is based on its central role in homologous recombination. The RecA protein binds strongly and in long clusters to ssDNA to form a nucleoprotein filament. The protein has more than one DNA binding site, and thus can hold a single strand and double strand together.
What is the role of RecA?
In addition to its role in homologous recombination, RecA functions as a coprotease for the LexA protein. In a healthy cell, LexA represses the expression of genes encoding DNA repair proteins (SOS genes). Upon injury of DNA, LexA catalyzes its own digestion, thereby allowing synthesis of necessary SOS proteins.
What is the meaning of RecA?
RECA
| Acronym | Definition |
|---|---|
| RECA | Radiation Exposure Compensation Act of 1990 |
| RECA | Responsible Electronic Communication Alliance |
| RECA | Responsible Energy Codes Alliance (Washington, DC) |
| RECA | Residual Capability Assessment |
What is the function of RecA protein in bacteria?
The bacterial RecA protein is the prototypical recombinase, promoting the central steps of DNA pairing and strand exchange in genetic recombination and recombinational DNA repair. RecA homologs are present in virtually all organisms from bacteria to humans.
What is a RecA mutation?
RecA filament catalyzes homologous strand exchange of the broken DNA duplex with an intact sister duplex, thus creating an opportunity for double-strand break repair (11). In recA or recBC mutants of E. coli, double-strand DNA breaks are not repaired (12) and cause chromosomal loss and cell death (13, 14).
What is RecA gene in bacteria?
Abstract. The RecA protein is a recombinase functioning in recombinational DNA repair in bacteria. RecA is regulated at many levels. The expression of the recA gene is regulated within the SOS response. The activity of the RecA protein itself is autoregulated by its own C-terminus.
What is RecA gene?
The recA gene product is a multifunctional enzyme that plays a role in homologous recombination, DNA repair and induction of the SOS response. . In homologous recombination, the protein functions as a DNA-dependent ATPase, promoting synapsis, heteroduplex formation and strand exchange between homologous DNAs. .
Is RecA an enzyme?
A key enzyme is RecA, which catalyzes the strand invasion process. RecA coats single-stranded DNA and facilitates its pairing with a double-stranded DNA molecule containing the same sequence, which produces a loop structure.
How is RecA regulated?
RecA is regulated at many levels. The expression of the recA gene is regulated within the SOS response. The activity of the RecA protein itself is autoregulated by its own C-terminus. RecA is also regulated by the action of other proteins.
