What is Anhydrotetracycline used for?

The transcriptional inducer anhydrotetracycline (aTc) and the bacteriostatic antibiotic tetracycline (Tc) are commonly used in all fields of biology for control of transcription or translation.

Is Anhydrotetracycline an antibiotic?

Product. Anhydrotetracycline is an antibiotic derivative of tetracycline. It is effective at lower concentrations than either tetracycline or doxycycline and is also less toxic to E. coli.

How do you dissolve Anhydrotetracycline?

A stock solution may be made by dissolving the anhydrotetracycline (hydrochloride) in an organic solvent purged with an inert gas. Anhydrotetracycline (hydrochloride) is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF).

What is Tet inducible system?

The Tet system is a conditional gene expression system where transcription is turned on or off in the presence of tetracycline or doxycycline. It affords very tight control over expression compared to other inducible systems and is reversible.

Does tetracycline have penicillin in it?

Tetracyclines are unrelated to penicillins and therefore are safe to take in hypersensitive patients. Other unrelated antibiotics include quinolones (e.g. ciprofloxacin), macrolides (e.g. clarithromycin), aminoglycosides (e.g. gentamicin) and glycopeptides (e.g. vancomycin).

What is ATc in microbiology?

We report the development of mycobacterial gene regulation systems that allow controlling gene expression in fast and slow-growing mycobacteria, including Mycobacterium tuberculosis, using anhydrotetracycline (ATc) as inducer.

What is Tet-On and Tet-Off system?

The Tet-Off and Tet-On systems are complementary, and the decision to choose one over the other depends on the particular experimental strategy. In the Tet-Off system, transcription is inactive in the presence of Tc or Dox; conversely, in the Tet-On system, transcription is active in the presence of Dox.

What does Tet-on mean?

The tetracycline-controlled Tet-Off and Tet-On gene expression systems are used to regulate the activity of genes in eukaryotic cells in diverse settings, varying from basic biological research to biotechnology and gene therapy applications.

What kind of infections does tetracycline treat?

Tetracycline is used to treat infections caused by bacteria including pneumonia and other respiratory tract infections; ; certain infections of skin, eye, lymphatic, intestinal, genital and urinary systems; and certain other infections that are spread by ticks, lice, mites, and infected animals.

Is it safe to take tetracycline?

Tetracycline is an antibiotic that treats many types of bacterial infections. It’s available as an oral capsule that’s safe to use in adults and children age 9 and older. Although it works well, it can cause unpleasant side effects and must be taken multiple times a day.

Why choose Takara Bio USA for your tetracycline-controlled gene expression system?

As the exclusive licensed distributor of tetracycline-controlled gene expression systems, only Takara Bio USA is certified to offer FBS that can ensure optimum induction with all tetracycline-inducible gene expression systems.

What is the difference between tetracycline and anhydrotetracycline?

Anhydrotetracycline is a derivative of tetracycline that exhibits no antibiotic activity. It is designed for use with tetracycline-controlled gene expression systems in bacteria. Anhydrotetracycline is an antibiotic derivative of tetracycline.

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