How is periodic acid formed?
How is periodic acid formed?
Periodates are made by oxidation of iodide, iodine, or iodate in aqueous solution. The industrial process involves oxidation of NaIO3 either electrolytically on a PbO2 anode (E1/2∘ = 1.6V, Fig. 1), or chemically using chlorine gas and sodium hydroxide.
What is periodic acid?
Definition of periodic acid : any of the strongly oxidizing acids (such as H5IO6 or HIO4) that are the most highly oxidized acids of iodine.
What is periodic acid solution?
The Periodic acid solution 0.5% for the PAS reaction (periodic acid Schiff) for the detection of aldehyde and mucosubstances in microscopy, is a ready to use solution used for the detection of mucopolysaccharides, glycogen, muco- and glycoproteins, glyco-, phospholipids, basal membranes and collagen.
What is periodic acid used for?
The periodic acid-Schiff (PAS) staining procedure is most commonly used in the histology laboratory to detect glycogen deposits in the liver when glycogen storage disease is suspected. Glycogen granules may also be visible in tumors of the bladder, kidney, ovary, pancreas, and lung.
What is the pH of periodic acid?
Pricing & Availability
| Physicochemical Information | |
|---|---|
| Melting Point | 122 °C |
| pH value | 1.2 (100 g/l, H₂O, 20 °C) |
| Bulk density | 1400 kg/m3 |
What is the role of periodic acid in PAS stain?
PRINCIPLE: The PAS stain is a histochemical reaction in that the periodic acid oxidizes the carbon to carbon bond forming aldehydes which react to the fuchsin-sulfurous acid which form the magenta color.
Why HIO4 is called periodic acid?
Periodic acid, or iodic acid is an oxoacid of iodine having chemical formula HIO4 or H5IO6. In dilute aqueous solution, periodic acid exists as discrete hydronium and metaperiodate ions. When more concentrated, orthoperiodic acid, H5IO6, is formed; this dissociates into hydronium and orthoperiodate ions.
Is periodic acid strong?
Periodic acid is a strong oxidizing agent, commonly used to oxidize glycols.
What is the principle of periodic acid Schiff?
What is the staining procedure of PAS stain?
Procedure of PAS Stain Oxidation: Place the sections in periodic acid solution (1%) for 5-10 minutes. Rinse: Wash in at least two changes of distilled water. Treatment with Schiff’s reagent: Cover with Schiff’s reagent for 20-30 minutes. Rinse: Rinse in running tap water for 5-10 minutes.
How do you make periodic acid?
Periodic acid was discovered by Heinrich Gustav Magnus and C. F. Ammermüller in 1833. Modern industrial scale production involves the oxidation of a solution of sodium iodate under alkaline conditions, either electrochemically on a PbO 2 anode, or by treatment with chlorine:
What are the different methods of preparing acids?
There are several methods for preparing acids. Some of them are discussed here. 1. Synthetic method : In the synthetic method, acids are prepared by direct combination of elements.
What is a periodic acid?
The periodic acid is any of the various iodine oxides that contain more oxygen than iodic acid and differ in water content, particularly one of the crystalline compounds, HIO 4 and H 5 IO 6. Periodic acid is defined as any acid-containing strongly oxidizing iodine, such as H 5 IO 6 or HIO 4.
What is the method of preparing hydrochloric acid?
Synthetic method : In the synthetic method, acids are prepared by direct combination of elements. For example, hydrogen and chlorine react together under the action of an electric spark to produce hydrogen chloride gas which is absorbed in water to give hydrochloric acid.
