What is the particle size of silica gel for column chromatography?

40-63 µm
Particle size While the most common silica particle size used in chromatography is 40-63 µm, we encourage you to contact us to discuss your specific application if you have any questions or concerns.

Why do silica gel columns with smaller particle sizes?

Particle size determines the efficiency of the silica material. Smaller particles produce more efficient separations. Small particles (< 2 µm) are most commonly used for high-resolution separations using longer columns or high-speed separations using shorter columns. Pore diameter is used to control retention.

What is the diameter of silica particle in HPLC?

Most reversed-phase HPLC columns are based on a stationary phase bonded to silica particles. These particles form the basis of the packing material. The most popular particle sizes are 5-, 3.5- and 3-μm particle diameters.

What does silica gel 60 mean?

The number “60” indicates that the mean pore diameter of the silica particles is 60 Е. This is the most commonly used silica in university and industrial laboratories.

What is mesh size in silica gel?

One typical particle size is 230-400 mesh (40-60 micron), but you don’t want to use silica with smaller particles because the flow through your columns will be too slow.

What is mesh size of silica?

technical grade, pore size 60 Å, 230-400 mesh particle size, 40-63 μm particle size.

Why is silica gel used in thin layer chromatography?

The Silica Gel G for Thin Layer Chromatography as binder is most extensively used as adsorbent in thin layer chromatography because of its excellent separation properties.

Why silica gel is used in column chromatography?

The most popular adsorbent used in column chromatography is Silica gel, primarily due to its huge affinity for adsorption. Moreover, it’s readily available in different variants, which makes it a commercially viable product for manufacturers.

What is particle size in HPLC?

The standard particle size for HPLC columns was 5 µm for a long time, until the mid-1990s, when 3.5 µm became popular for method development. More recently, as higher speed and/or higher resolution is required, chromatographers have turned to packings with sub-2-3 µm, including 1.8 µm.

How does particle size affect chromatography?

Smaller particle size improves efficiency of a separation without increasing run time, column length, or flow rate. The arrows illustrate regions of the chromatograms where the increase in efficiency and resolution is significant.

What is G in silica gel?

The “G” stands for Gypsum and indicates that calcium sulfate hemihydrate (CaSO4·1/2H2O), a refined form of gypsum, has been included in the adsorption formulation. Gypsum functions as a binder, improving the cohesion of the adsorbent particles and increasing the adhesion of the layer to the glass substrate.

What is the density of silica gel?

700 kg/m³Silica gel / Density

What is the particle size of irregular silica gel?

Irregular Silica Gels Portfolio by Particle Size. 5 – 20 µm 15 – 40 µm 20 – 45 µm 40 – 63 µm 60 – 120 µm 60 – 200 µm 75 – 150 µm 75 – 250 µm 105 – 175 µm 120 – 200 µm 150 – 250 µm 200 – 500 µm 250 – 500 µm 500 – 1000 µm 800 – 1200 µm. With particle sizes from 5 μm up to 1200 μm, SiliCycle offers irregular silica gels that will meet all your

What is silica gel?

Silica gel was in existence as early as the 1640s as a scientific curiosity. It was used in World War I for the adsorption of vapors and gases in gas mask canisters. The synthetic route for producing silica gel was patented in 1918 by Walter A. Patrick, a chemistry professor at Johns Hopkins University .

What is the size of silica gel in column chromatography?

In chemistry, silica gel is used in chromatography as a stationary phase. In column chromatography, the stationary phase is most often composed of silica gel particles of 40–63 μm. Different particle sizes are used for different kinds of column chromatography as the particle size is related to surface area.

What is the adsorption capacity of silica gel?

Silica gel is a suitable adsorbent for water, alcohol, phenols, amines, and so on. That does this through hydrogen bonds. The adsorption capacity of silica gel at low temperatures is higher than that of alumina and zeolite; however, if the moisture content is low, it is less adsorbent than zeolite.