Does GFP antibody recognize mCherry?

Does GFP antibody recognize mCherry?

Q: Does this antibody cross react with dsRed or mCherry? A: The anti-GFP antibody was made using the N-terminal of GFP as an antigen and will not react with RFP proteins dsRED or mCherry, since their sequences are so different.

Can I use mCherry and GFP?

Due to the spectral properties of GFP and mCherry, they are considered an ideal combination for co-localisation and co-expression experiments.

What is mCherry antibody?

mCherry is a fluorescent protein derived from Discosoma sp. Mushroom corals.

Does GFP antibody recognize CFP?

Specificity. GFP antibody (ab6556) is reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP, RFP and EGFP.

Does GFP antibody recognize RFP?

All Rockland RFP antibodies are affinity-purified assuring both high affinity and specificity and have been pre-absorbed to eliminate any potential cross-reactivity to human, mouse and rat serum proteins. The antibodies are also confirmed for non-reactivity to GFP protein.

Is mCherry a dimer?

If mCherry exists in a single brightness state, the brightness of the dimer is expected to be twice that of the monomer, as has been confirmed for the proteins EGFP (15) and EYFP (see Table S1 of the Supporting Material).

Does DsRed antibody recognize mCherry?

Panel A. The mCherry Monoclonal Antibody recognizes mCherry but not tdTomato, DsRed-Express2, or DsRed-Monomer.

What is anti GFP?

Anti-GFP antibody (ab290) is a highly versatile antibody that gives a stronger signal than other anti-GFP antibodies available. On Western blot the antibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins and has also been shown to be useful on mouse sections fixed with formalin.

How does red fluorescent protein work?

Red fluorescent protein (RFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. RFP can be excited by the 488 nm or 532 nm laser line and is optimally detected at 588 nm.