What is the Langendorff isolated heart preparation?

In the Langendorff preparation, the heart is first surgically removed from the animal’s body. Subsequently, the aorta is suspended in the Langendorff apparatus and the heart is perfused via the aorta, usually with a nutrient rich oxygenated solution [Krebs and Henseleit solution (KHB)].

What is Langendorff perfusion system?

The Langendorff Perfused Heart Model is an experimental procedure developed at the end of the nineteenth century by Oskar Langendorff. In this procedure, an excised heart has a cannula inserted into its aorta so that the heart can be retrogradely perfused via the coronary artery.

What is a perfused heart?

Definition. The isolated perfused heart according to Langendorff is a preparation which is easily obtained from all warm-blooded animals. The heart is usually perfused at constant pressure, but frequently at constant flow, mostly with oxygenated saline solutions.

What is the purpose of perfusing solution into the aorta in the direction of the heart?

The basic principle is to maintain cardiac activity by perfusing the heart via the coronary arteries using an aortic cannula inserted into the ascending aorta. Perfusion solution is delivered to the heart in a retrograde manner via this cannula.

What is Langendorff preparation?

In the Langendorff preparation, the heart is removed from the animal’s or human’s body, severing the blood vessels; it is then perfused in a reverse fashion (retrograde perfusion) via the aorta, usually with a nutrient rich, oxygenated solution (e.g. Krebs–Henseleit solution or Tyrode’s solution).

What is Langendorff apparatus?

The Langendorff Apparatus & Thermostat Controller is a compact, self-contained and thermostatically-controlled unit, which is ideal for the retrograde perfusion (Langendorff) of isolated small rodent (mice and rats) hearts.

What is langendorff apparatus?

What is langendorff preparation?

Why doesn’t solution enter the left ventricle when it is perfused into the aorta by the Langendorff technique?

Unlike the working heart model, the Langendorff system relies on retrograde perfusion of the heart (through the coronary vasculature) in order to maintain cardiac function. This means the perfusate does not enter the left ventricle.