What is glycan mapping?

The Agilent AdvanceBio Glycan Mapping workflow provides a timesaving, optimized method to generate glycosylation profiles for these important biotherapeutics. The glycan structure attached to the protein during glycosylation is particularly important because it affects biological activity.

What is the difference between glycan and glycosylation?

Glycosylation (see also chemical glycosylation) is the reaction in which a carbohydrate (or ‘glycan’), i.e. a glycosyl donor, is attached to a hydroxyl or other functional group of another molecule (a glycosyl acceptor) in order to form a glycoconjugate.

What are glycan receptors?

Glycan-binding receptors that selectively remove glycoproteins from blood. (A) Receptors in the liver. The mannose receptor on liver sinusoidal endothelial cells and Kupffer cells binds to glycoproteins that constitutively display glycans that signal rapid clearance from the circulation.

What are N and O-glycans?

N-glycans are typically released from glycoproteins by enzymes, while O-glycans are released from glycoproteins by chemical methods. It is important to identify and quantify both N- and O-linked glycans of glycoproteins to determine the changes of glycans.

How is glycan analysis done?

Glycan structure assessment is challenging due to the isomeric and branched nature of oligosaccharides. The traditional approach involves the use of exoglycosidases to selectively and sequentially release terminal monosaccharides, which produces trimmed glycans to be analyzed by HILIC, CE or MALDI-MS.

What is the difference between glycan and polysaccharide?

Polysaccharides and oligosaccharides are also known as glycans. Glycans usually possess O-glycosidic linkages between monosaccharides. Cellulose, for example, is a glycan with β-1,4-linked D-glucose. Chitin is another glycan with β-1,4-linked N-acetyl-D-glucosamine.